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Enclosure 2- San Miguel River instream Flow Recommendation <br />Flow Quantification Methodology <br />PHABSIM and R2Cross Methodology <br />The Colorado Division of Wildlife (CDOW) and the Bureau of Land Management (BLM) <br />evaluated all of the data collected to date and determined that best flow recommendation would <br />come from using the results from a combination of methods. Physical Habitat Simulation <br />(PHABSIM) is a widely accepted method for quantifying the suitable versus unsuitable hydraulic <br />habitat attributes of selected species and life stages as a function of discharge. The R2CROSS is <br />best suited for identifying flows with specific hydraulic criteria across riffle type habitats. The <br />State of Colorado has used the R2CROSS extensively in the past to appropriate instream flow <br />water rights. The CDOW and the BLM were concerned that exclusive use of the standard <br />R2CROSS method may not be appropriate for this reach of the San Miguel River due to its <br />major width (over 75' wide in most places), type of fish species present (warm /cool water <br />species) and its big river channel hydraulics and characteristics. <br />The CDOW and the BLM collected transect and flow data for seven different cross- sections <br />within an 815 -foot reach of stream. The transect data was collected at a site approximately 1.5 <br />miles upstream from the confluence of the San Miguel River with Tabeguache Creek. These <br />seven transects incorporated different mesohabitat types including riffles, runs, pools and glides. <br />These seven different cross - sections formed the basis for the PHABSIM /River Habitat <br />Simulation ( RHABSIM) study conducted by the CDOW and the BLM. The PHABSIM is <br />widely used in North America to quantify instream flow regimes and consists of two modeling <br />components, <br />These initial recommendations are designed to address the unique biologic requirements of this <br />stream reach without regard to water availability. In addition to the criteria developed using the <br />PHABSIM Methodology and the RHABSIM Software, the three standard instream flow <br />hydraulic parameters used in the R2CROSS (average depth, percent wetted perimeter and <br />average velocity) were also used to calculate and predict the biologic instream flow <br />recommendations. <br />Application of Habitat Suitability Criteria <br />Habitat Suitability Criteria (HSC) were developed from the 2003 Riverine Fish Flow <br />Investigation Study Report (Federal Aid Project F- 289 -R6) written and performed by Richard <br />Anderson, CDOW Aquatic Researcher, and Gregory Stewart, Department of Geosciences <br />Oregon State University'. The basis for this study was a 1999 request from the Colorado Water <br />Conservation Board (CWCB) for the CDOW to provide biologically justified instream flow <br />1 See "Impacts of stream flow alterations on native fish abundance and native fish habitat and the use of native fish <br />population data to support instream flow recommendations made using a 2D instream flow methodology." <br />