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Task 1.1 - Field Lead, togistics, sub-project lead -sampling water for nutrients and <br />bacteria <br />At each sample site, water will be collected and analyzed for nutrients (nitrate + nitrite, <br />total Kjeldahl nitrogen, ammonia, totat phosphorus, and orthophosphate) pH, <br />conductivity, and E. coli bacteria and the ftow for each will be measured in order to <br />complete a loading analysis. The same efforts will occur for the Animas River upstream of <br />each return ftow. <br />Subtask 1.2 - Levei 1 Technician for benthic macroinvertebrate sampling <br />Macroinvertebrates are indicators of the long term health of the river and of each reach <br />and will also be collected at each sample site and analyzed using standard methods. <br />Subtask 1.3 - Lab identification of benthic macroinvertebrates <br />Macroinvertebrate community composition and other metrics are excellent indicators of <br />the health of a river and they have been used as indicators throughout SW Cotorado. In <br />the lab macroinvertebrates samples witl be processed and identified and metrics <br />calculated and analyzed compared to regional data. <br />Subtask 1.4 Total nitrogen and total phosphorus (nutrients) tab processing <br />Analysis of water samples collected at each sample site for TN and TP. <br />Subtask 1.5, E, coli bacteria lab processing <br />Processing field samples for E.coli bacteria, indicators of hyperenrichment of streams <br />Subtask 1.6 - Data Analysis £t Reporting on nutrients, bacteria, macroinvertebrates <br />Utilizing the extensive database for macroinvertebrates, bacteria and water quality, the <br />Animas River and inflows will be characterized in relation to other streams and rivers as <br />wett as to other sample sites on the Animas River. Identification of the overatl quality of <br />water of the Animas River between Baker's Bridge and the New Mexico State Line and <br />surface sources of nutrient loads to the Animas will be the primary deliverables of this <br />task. <br />Task 2- Periphvton Biomass and Nitro�en 15 Stable Isotope <br />Analyses <br />In this task, periphyton samples will be collected and the biomass of the periphyton will <br />be measured and anatyzed for nitrogen 15 stable isotope signatures. Periphyton are the <br />algae, bacteria, and fungi growing on surfaces in an aquatic environment. This task witl <br />indicate areas where untreated or poorly treated sewage enters the river either through <br />poorly maintained or poorly engineered treatment plants and/or septic systems and <br />where non-point sources of pollution and poor assimilation capacity of the river may be a <br />factor. <br />Subtask 2.1 - Subproject lead - periphyton sampling <br />Rocks will be collected from riffles at approximately 35 locations at roughly one mile <br />intervals along the Animas River main-stem. Four rocks will be collected from four <br />locations wifhin the riffle to gain a more representative profile of a given riffle. Rocks <br />will be scraped and scrubbed to remove periphyton from a standard area of 10 cm <br />2 <br />