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DESIGNING IMPACT ASSESSMENTS FOR EVALUATING ECOLOGICAL EFFECTS OF AGRICULTURAL CONSERVATION PRACTICES ON STREAMS <br />large geographic areas to provide a time series repre- <br />senting the status of the target/populations and habi- <br />tat conditions. Conversely, impact assessments are <br />conducted at a variety of spatial and temporal scales <br />depending on the research hypothesis and funding <br />base (Engel and Voshell, 2002). Impact assessments <br />with large spatial and temporal scales are considered <br />the most robust and necessary for evaluating the <br />impacts of conservation and restoration practices <br />(National Research Council, 1992; Kondolf, 1995), but <br />smaller scale studies can provide valuable informa- <br />tion as long as their limitations are recognized. <br />Differences between monitoring studies and impact <br />assessments highlight the need for guidance in <br />designing impact assessments. Our objective is to <br />provide five guiding principles that will assist others <br />in designing impact assessments of ecological <br />responses of agricultural streams to conservation <br />practices. We first provide background information <br />and describe the challenges faced in developing the <br />guiding principles. Second, we explain each principle <br />and defend their importance. We conclude with an <br />example of how the guiding principles were applied <br />in designing an evaluation of the influence of herba- <br />ceous riparian buffers on the habitat and aquatic <br />communities within channelized headwater streams <br />(i.e., drainage ditches). <br />TOWARDS THE DEVELOPMENT OF <br />GUIDING PRINCIPLES <br />The guiding principles below resulted from an <br />attempt to develop standardized sampling protocols <br />for the Agricultural Research Service (ARS) compo- <br />nent of the Conservation Effects Assessment Project <br />(CEAP). The ARS component of CEAP consists of the <br />Watershed Assessment Study that is intended to pro- <br />vide detailed findings on the influence of conservation <br />practices from 14 watersheds in the U.S. (Mausbach <br />and Dedrick, 2004) (Figure 1). The ARS research <br />effort focuses mostly on evaluating water chemistry <br />and hydrological responses to conservation practices. <br />Ecological responses are currently being evaluated in <br />only three watersheds (Figure 1) despite the critical <br />need for this information due to the elevated threat <br />level currently faced by stream ecosystems (Richter <br />et al., 1997; Karr et al., 2000). Additionally, the <br />national assessment of conservation practices as part <br />of CEAP plans to use watershed models (Mausbach <br />and Dedrick, 2004) to evaluate water chemistry and <br />selected hydrological responses to conservation prac- <br />tices (Shields et al., 2006a). These models are not <br />capable of evaluating physical habitat responses (i.e., <br />FIGURE 1. Locations of Agricultural Research Service Conserva- <br />tion Effects Assessment Project Watershed Assessment Study <br />Watersheds Within the United States. Watersheds symbolized by a <br />black circle within a white circle are study sites where ecological <br />assessments are being conducted. <br />base -flow discharge, substrate types, large woody deb- <br />ris, water temperature) or biological responses (i.e., <br />fish and macroinvertebrate community responses) <br />(Shields et al., 2006a). Ecological assessments (i.e., <br />joint assessments of habitat and biological responses) <br />have the potential to provide information for novel <br />developments in these watershed models that would <br />allow the national assessment of conservation prac- <br />tices to conduct a cross - disciplinary assessment of <br />conservation practices through the evaluation of <br />water chemistry, hydrology, physical habitat, and <br />ecological responses. We initiated the development of <br />a standardized protocol to facilitate the: (1) initiation <br />of ecological research within ARS watersheds, (2) col- <br />laborative ecological research among watersheds, and <br />(3) cross - disciplinary assessments of conservation <br />practices. <br />Developing a standardized sampling protocol <br />immediately proved to be a challenge. Twelve of the <br />ARS watersheds are lotic ecosystems, while two of <br />the watersheds are lentic ecosystems. Watershed <br />sizes range from 850 to 827,000 ha. The watersheds <br />are located in 11 states within the Northeast, Mid- <br />west, Southeast, and Northwest U.S. and encompass <br />11 ecoregions ranging from the Middle Atlantic <br />Coastal Plain to the Northern Basin and Range Ecog- <br />regions (Omernik, 1987, 1995). The amount of crop- <br />land within each watershed ranges from 2 to 100% <br />and land use in seven watersheds is predominately <br />cropland ( >_60 %). Six of the ARS studies are evaluat- <br />ing the influence of nutrient management and/or <br />riparian buffers. However, research within 13 ARS <br />watersheds also includes assessments of one to three <br />other conservation practices from among 12 types <br />ranging from manure management to drainage <br />JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION 869 JAWRA <br />