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Appendix Table 3. <br />Dexter National Fish Hatchery and Technology Center <br />` 81 Razorback Sucker Spawning Protocols: 2003 <br />Roger Harman <br />screen, board and start filling pond IA March 21, 2003 <br />-check eggs in incubators <br />March 15, 2003 -individual egg lots can be moved at this time <br />-start draining pond 6A -prepare a minimum of two 12' tanks to <br />receive fry <br />March o screen, 2003 <br />March 16, 2003 <br />-continue draining pond 6A <br />.finish draining pond 6A and bring all <br />March 17, 2003 <br />broodstock to Fish Culture Building <br />-sort males/females and place in separate <br />tanks <br />-record pit tag numbers, lengths, weights <br />and take genetic samples of each fish <br />-inject 25 females with O.lcc HCG/lb in <br />preparation for spawning <br />-inject 25 males if necessary with 0.3 cc <br />HCG/lb in preparation for spawning <br />-take remaining broodstock not used in <br />spawning trials to pond IA <br />March 18, 2003 <br />-inject 25 females with O.lcc HCG/lb <br />March 19, 2003 <br />-inject 25 females with 0.1cc HCG/lb <br />-prepare incubation system to receive eggs <br />-gather other equipment and supplies needed <br />for spawning trials <br />March 20, 2003 <br />-spawn razorbacks using 1 female X 1 male <br />spawning procedure <br />-inventory each individual spawn <br />-place eggs in incubators <br />-take fish used in spawning trails to pond IA <br />-subset of each spawn for broodstock <br />development to aquaria for hatching <br />March 22, 2003 <br />-check eggs in incubators <br />March 23, 2003 <br />-check eggs in incubators <br />-commence filling 12' tanks with heated <br />water <br />March 24, 2003 <br />-check incubators (morning and afternoon) <br />and transfer fry to 12' tanks <br />March 25, 2003 <br />-check incubators (morning and afternoon) <br />and transfer fry to 12' tanks <br />March 26, 2003 <br />-transfer remaining fry to 12' tanks <br />-clean incubators <br />March 27, 2003 <br />-observe fry in 12' tanks <br />March 28, 2003 <br />-observe fry in 12' tanks <br />March 29, 2003 <br />-observe fry in 12' tanks <br />March 30, 2003 <br />-observe fry in 12' tanks <br />-clean 12' tanks in preparation for shipment <br />March 31, 2003 <br />-fry can be shipped at this time to meet <br />production commitments <br />-all fry shipped no later than April 4, 2003 <br />54