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Management Action: When more than 10,000 fish are needed for a production <br />commitment, a two-by-two mating scheme will be employed, with a minimum of ten <br />pairs. <br />A. Task: spawn from two females will be split, and two males will be used to <br />fertilize the eggs, each male fertilizing % of each female's spawn. This will result <br />in four % sibling families, as opposed to two full sibling families. <br />Target date: February 2004 <br />B. Task: when parents are used in a one on one paired mating production strategy, <br />or two by two strategy, contributions of each cross will be equalized. Multiple <br />stocking commitments can be met from the same matings, but no more than 5,000 <br />fish will be stocked in any one area from one fish. Excess eggs from each paired <br />mating will be maintained for research purposes, sent to other facilities or disposed <br />of. <br />Target date: February 2004-July 2008, <br />C. Task: Broodfish will be rotated so that no fish will be spawned twice in five <br />years. <br />Target date: February 2004-July 2008 <br />D. Task: Expected allelic frequencies will be developed for each paired mating, <br />and for each pool of production fish. Target date: July 2005-July 2008 <br />6) Establish breeding program, using the database to maintain and ensure that the effective <br />population size of broodstock is adequate to offset the results of small populations: <br />Management Action: Maintain at least 3,000 adult broodstock at Dexter NFHTC. <br />35