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8/11/2009 11:32:58 AM
Creation date
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UCREFRP
UCREFRP Catalog Number
9716
Author
Stone, D., and P. Sponholtz.
Title
Translocation of young-of-year humpback chub above Chute Falls in the Little Colorado River, AZ, 2003 Interim Report.
USFW Year
2003.
USFW - Doc Type
U.S. Fish and Wildlife Service
Copyright Material
NO
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from the system and larger-bodied predators examined for stomach contents. Trammel <br />nets, seining, and angling were also attempted, but were abandoned because of high loads <br />of drifting algae (trammel nets), rocky and algal covered bottoms (seining), and the low <br />numbers of large-bodied fish (angling). We recorded visual observations of large-bodied <br />nonnative fishes. <br />Because high levels of dissolved CO2 have been considered a potential deterrent to <br />humpback chub residing in this region (Mattes 1993; Strength 1997), we collected samples <br />at eight separate locations from below Upper Atomizer Falls (13.87 RKM; where humpback <br />chub have been historically captured) to Blue Springs (20.72 RKM). These measurements <br />were taken by titrating LCR water mixed with phenolphthalein indicator with 3.636 N <br />Sodium Hydroxide using a HACH digital titrator (Model 16900-01; Hach Co., Loveland, <br />CO). We used a graduated cylinder to collect water and decant it into disposable clear <br />plastic cups for each individual titration. This was to alleviate problems associated with <br />contamination resulting from residue Sodium Hydroxide and CaCO3 precipitation. We <br />collected at least five samples at each site, whereby the highest and lowest titration was <br />discarded (i.e., greatest outliers) before taking means of the samples. We also measured <br />water temperatures at each site because of its influence on CO2. Water turbidity <br />measurements (mean nephelometric turbidity units "NTU", Hach Model 2100P <br />Turbidimeter, Loveland, CO) were taken daily at 14.7 RKM. <br />Only one potential helicopter landing site located at 16.2 RKM was considered suitable <br />during flood conditions (Figure 1). Although numerous sandy beaches were available, they: <br />could easily become inundated under higher flows. A comparison between our map with <br />that of Robinson et al. (1996) revealed that they had previously used this site (i.e., their <br />second lowest site) to examine the survivorship and behaviors of humpback chub in June <br />1994. This site was ideally located to translocate, acclimate, and release humpback chub <br />within a large vegetated pool. In addition, this area was where Robinson et al. (1996) <br />believed that humpback chub could tolerate the CO2 concentration, and was >2 RKM <br />above Chute Falls. The site also offered personnel high-ground protection from floods. <br />Translocation Trip <br />We conducted the humpback chub translocation trip from July 28 -August 1, 2003. On the <br />first day two biologists flew to Boulder Camp (1.9 RKM) and deployed 14 baited hoopnets <br />between 1.8 to 2.6 RKMs, while the other two biologists flew to the 16.2 RKM release site <br />to construct a helicopter landing pad. We pulled all nets the following day and transported <br />50 -100 mm humpback chub back to camp, where they were placed in holding nets <br />secured in the LCR, and left undisturbed to digest consumed bait until the following evening <br />(>24 hours post-capture). On July 30, 2003, 300 humpback chub were measured (TL; <br />mm), anaesthetized with tricaine methanesulfonate (MS-222), implanted with yellow <br />elastomer tags near their dorsal fins on the left side (for later verification purposes), and <br />allowed to recover overnight in an aerated transport tank. On July 31, 2003 the transport <br />tank was set to deliver 3 liters/minute oxygen and slung by helicopter to the 16.2 RKM <br />release site. At the release site, we slowly tempered the humpback chub by exchanging <br />1 /3 of the oxygenated holding-tank water with fresh LCR water every 15 minutes until CO2 <br />7
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