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Genetic variation as measured by heterozygosity for all populations surveyed was <br />higher than the mean estimate for freshwater fish in general; and. mean allelic richness <br />over all loci for all chub populations fell within one standard deviation of the mean for <br />freshwater species. The Moapa River population had the lowest values of mean allelic <br />richness (A = 5.34), expected heterozygosity (HE = 0.56) and numbers of unique alleles. <br />The Dexter populations had similar levels of genetic variation (A ranged from 6.11 to <br />6.28; HE = 0.67) in comparison to the Virgin River populations (A ranged from 6.15 to <br />6.22; HE ranged from 0.67 to 0.68). <br />Introduction: <br />The Virgin River chub (Gila seminuda) is a member of the minnow family <br />(Cyprinidae) that averages 20 cm (8 inches) in total length (USFWS 1994). <br />Although originally treated as a subspecies of G. robusta, Virgin River chub was later <br />determined to be a full species of hybrid origin (DeMarais et al. 1992). Historically, <br />Virgin River chub was found in the Moapa River, NV, and in the mainstem Virgin River <br />from Pah Tempe Springs, Utah downstream to the confluence with the Colorado River in <br />Nevada (Cope and Yarrow 1875, Cross 1975, as cited by USFWS 1994). At <br />present, Virgin River chub still occur in these waters, however, the species distribution in <br />the Virgin River has diminished notably. Virgin River chub occurs only from Pah Tempe <br />Springs, Utah to the Mesquite Diversion near the border of Arizona and Nevada. The two <br />core areas thought to contain the largest existing populations include: the 7.5 mile reach <br />of the mainstem from the State Route 9 bridge near LaVerkin to just below Gould's <br />Wash in Utah, and the 5.5 mile reach from the lower mouth of the Virgin River Gorge to