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<br />PERFORMANCE OF PIT TAG INTERROGATION SYSTEMS <br /> <br />405 <br /> <br />Rattlesnake Creek project, we used three tag models <br />produced by Digital Angel Corporation: BE (n = <br />1,343) at the beginning, ST (n = 2,566) in the middle, <br />and SGL (n = 702) at the end of the study. Each <br />subsequent tag had better read ranges than the former. <br />At low EMF noise levels and optimum orientation, the <br />ST model had up to 42% better read range than the BE <br />tag (Peterson Engineering Services 2002), and the SOL <br />tag had up to 9.3% better read range than the ST tag <br />(Downing et al. 2005). As stated by Zydlewskiet al. <br />(2006), these increases in read nmge are relatively <br />small compared with what would be expected from the <br />use of the next larger size of PIT tag (i.e., 23 mm in <br />size). . <br />Tagging in Beal'er Creek.-A total of 3,913 rainbow <br />trout, steelhead, and brook trout (FL: range = 65-760 <br />mm, mean = 120 mm, median = 115 mm, SD = 52.9) <br />were PIT-tagged (1,672 ST tags and 2,241 SOL tags) <br />in the Beaver Creek watershed in 2004 and 2005. <br />Juvenile steel head and rainbow trout (n = 3,230) made <br />up the majority of the tagged fish (79%). We used <br />electrofishing gear to collect fish throughout the <br />watershed of Beaver Creek and a two-way fish trapping <br />weir located at rkm 1. Most of the electrofishing <br />occurred during the summer, spring, and fall. The weir <br />was operational from 22 October through 22 December <br />in 2004 and from 20 March through 5 December in <br />2005. <br />Installation and configuration of interrogation <br />systems.-ln both Rattlesnake and Beaver creeks, we <br />installed a custom-made PIT tag interrogation system <br />to monitor fish movement. We needed a system that <br />could be deployed in a natural section of stream, could <br />distinguish between downstream and upstream move- <br />ments, and would not need daily attention like a weir or <br />trap. Each interrogator had six antennas arranged in <br />three arrays of two antennas each (i.e., a 3 X 2 design). <br />When a tag was detected, these systems provided <br />information on what lmtenna it was read on and the <br />date and time that the detection occurred. <br />The PIT tag interrogation system in Rattlesnake <br />Creek was installed at rkm 0.2. just upstream of its <br />confluence with the White Salmon River. Although <br />some antennas were installed in August 2001 (Con- <br />nolly et al. 2005), it was not until 2003 that we <br />acquired a multiplexing transceiver, Digital Angel's <br />Model FSlOOIM, capable of autotuning and operating <br />up to six antennas. Subsequently, we designed and <br />installed an interrogation system with three arrays of <br />two antennas each. The antennas were systematically <br />nwnbered in a successively downstream manner, river <br />left to river right (Figure 2). The transceiver wa~ <br />located in a weatherproof housing near the stream. The <br />FSlOOIM transceiver operated on 24-V DC power. <br /> <br /> Flow <br /> ! <br />I Ant. #2 II Ant. #1 I Array A <br />Pass-bys <br /> <br />Ant. #4 II <br /> <br />Ant. #3 <br /> <br />Array B <br />Hybrids <br /> <br />Ant. #6 II <br /> <br />Ant. #5 <br /> <br />Array C <br />Pass-bys <br /> <br />FiGURE 2.-A conceptual diagram of the three-aIT'dY, six- <br />antenna PIT tag interrogation system used in Rattlesnake and <br />Beaver creeks. Pa~s-by antennas were anchored to the <br />substrate at all four comers; hybrid antennas (Ant.) were <br />anchored on the upstream side so that the downstream side <br />could pivot up in the water column. <br /> <br />This power was provided by a 24-V AC-to-DC linear <br />power supply, which was cOlmected to grid power. <br />Antennas were constructed with polyvinyl chloride <br />(PVC) pipe to create rectangular shapes that varied in <br />length and width. The antennas (numbers I and 2) in <br />the upstream-most array (array A) each measured 3.1 m <br />X 0.6 m. The middle array (array B) had a river left <br />antenna (number 3) that measured 3.1 m X 0.6 m and a <br />river right antenna (number 4) that measured 2~0 m X <br />0.8 m. The downstream-most array (array C) had a <br />river left antenna (number 5) that measured 3.1 m X 0.6 <br />m and a river right antenna (number 6) that mea~ured <br />2.0 m X 0.8 m. By varying the lengths of the antennas, <br />we were able to span. mosl of the low-flow wetted <br />width, thalweg, and one stream bank with a single <br />antenna, and by adding a second antenna, we were able <br />to include all or some of the stream's bank-full width. <br />We used two methods to attach the antennas to the <br />substrate to maximize the detection of PIT -tagged fish <br />and the probability that the antennas would function <br />during a dynamic range of stream flows. Antennas <br />within the upstream-most (array A) and downstream- <br />most (array C) arrays (Figure 2) were attached at all <br />