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<br />razorback suckers were added, and observation resumed. This experiment ran for 13 days. The third trial <br />was similar to the previous test; the same two flathead catfish were used and 10 new razorback suckers <br />were added. However, the sanctuary zone was reduced to one-fourth of the tank's area (fig. 5) For four <br />days, we periodically recorded where fish were observed in the tank. <br /> <br /> <br />Figure 5. Tank configuration lone-fourth sanctuary) used for trial 3. The divider was placed along the <br />center of the tank, dividing it into equal halves for trials 1 and 2. <br /> <br />Statistical Analysis <br /> <br />Razorback Sucker Predation Trials <br />Fish treatment varied between years: in 2006, treated fish were exercised only; in 2007, treated <br />fish were exercised and exposed to a predation event. We were interested if treated fish were preyed <br />upon less than control fish (Ha' predation was random and mortality of treatment and control fish was <br />equal; HA, predation was not random and mortality of treatment fish was less than mortality of c011lrol <br />fish). <br />The number of predation trials in both years had relatively small sample sizes and values were <br />often small and discrete (for example, number of fish eaten was <5 in many trials). Therefore, we used <br />nonparametric tests to compare mortality of razorback suckers: either the Wilcoxon Matched Pairs <br />Ranks test or Multi-Response Permutation Procedure (MRPP) Matched Pairs test. Significance was <br />determined at the 0.05 level for all tests on predation. <br /> <br />Bonytail Predation Trials <br />We examined predation of bony tail in small arena experiments using groups of two to three <br />bony tail per treatment. A trial ended as soon as a predation event was observed, and we deterrnJl1cJ II <br />the consumed fish was a treatment or control. We examined the binomial proportion of trial success <br />using a Goodness of Fit test (HO, predation was random and ratio of trials was I: 1 ; HA, predation was <br />not random and treatment fish succeeded in more trials than control fish). <br /> <br />8 <br />