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IF <br />938 <br />MODDE ET AL. ; <br />emerge and enter the water column during peak <br />discharge when floodplain nursery habitats are <br />connected to the river, allowing larvae to drift into <br />nursery habitats (Modde et al. 2001). In addition, <br />postspawning adults also access nutrient rich <br />floodplains following spawning (Modde and Ir- <br />ving 1998). Thus, locality and timing of razorback <br />sucker spawning are critical and related to hydro- <br />logical events linked to specific life history needs. <br />The recovery plan proposed for razorback suck- <br />ers (USFWS 2002) includes instream flow rec- <br />ommendations requiring minimum flows and ma- <br />nipulating the frequency and timing of discharge <br />to increase floodplain connection at the time when <br />larvae are present (Muth et al. 2002), as well as <br />population augmentation (Nesler et al. 2003). The <br />augmentation plan states that 92,500 subadult <br />(>300 mm total length [TL]) razorback suckers <br />will be stocked over a 5-year period, the goal being <br />to establish a population of 7,540 adults (Nesler <br />et al. 2003). If the number of adult spawners, and <br />thus reproduction, are increased, flow recommen- <br />dations will reconnect nursery habitat to the river <br />and allow access to critical habitat by drifting lar- <br />vae, which should thereby increase the probability <br />of their survival and successful recruitment. How- <br />ever, the ability of stocked fish to contribute to <br />reproduction requires that they are able to locate <br />specific spawning sites upstream of nursery hab- <br />itat. Scholz et al. (1996) provided experimental <br />data suggesting both razorback suckers and Col- <br />orado pikeminnows Pytchocheilus lucius imprint <br />to emergence sites before larval drift. If for this <br />or other reasons hatchery fish introduced into the <br />system are not able to find established spawning <br />sites, the benefits of augmentation will be limited <br />and may even adversely affect recovery if hatchery <br />fish compete for resources with wild fish without <br />contributing offspring. The purpose of this study <br />was to examine whether hatchery-reared fish will <br />show similar spatial and temporal use of a spawn- <br />ing area used by wild fish and to examine the po- <br />tential importance of the results to the recovery <br />effort. <br />Methods <br />Study area.-The study area is the primary <br />spawning location for razorback suckers in the <br />middle Green River, Utah, located at approxi- <br />mately river kilometer (rkm) 504 (river course dis- <br />tance upstream of the confluence with the Colo- <br />rado River). This site, referred to as the Escalante <br />(Modde et al. 1996) or Jensen (Tyus and Karp <br />1990) spawning site, is located along the eastern <br />Cub Creek <br />Uta <br />h ,4! <br />der <br />QL <br />Spawning <br />rkm 505 <br />`Data Logger <br />FIGURE 1.-Map of the study area showing the spawn- <br />ing aggregation site for razorback suckers in the middle <br />Green River, Utah (upper Colorado River basin) at river <br />kilometer (rkm) 504. <br />channel, where the river moves around a large is- <br />land (Figure 1). Razorback suckers congregate an- <br />nually along a cobble-gravel bar at the initiation <br />of spring flood flows (Tyus and Karp 1990; Modde <br />and Irving 1998). <br />Collection of razorback suckers.-Razorback <br />suckers were captured by electrofishing (Smith- <br />Root GGP transducer mounted in a johnboat). <br />Three to six passes were made over the spawning <br />bar weekly from 1 to 22 May 2000. Each fish <br />captured was examined for a passive integrated <br />transponder (PIT) tag and gender (i.e., presence of <br />tubercles, expressing milt or eggs) and weighed <br />(0.1 g) and measured (TL; mm). Fish without a <br />PIT tag were tagged, and records were consulted <br />to determine the origin of fish with PIT tags (i.e., <br />wild or hatchery produced). As part of an inde- <br />pendent recovery effort to develop a genetic re- <br />fugia and broodstock for future stocking (i.e., Up- <br />per Colorado River Basin Endangered Fishes Re- <br />covery Program), all wild females and a limited <br />number of wild males were removed from the river <br />and transported to Ouray National Fish Hatchery <br />(NFH). <br />Attachment of radio transmitters.-Radio trans- <br />mitters were externally attached to razorback suck- <br />ers collected from the spawning area (as per Muell- <br />er and Marsh 1998; Mueller et al. 2003). We used <br />coded transmitters with a 149-Mhz frequency and <br />a 44-d life span, weighing approximately 6.5 g <br />(Lotek Engineering Inc.). Each transmitter was at- <br />tached to the keel (anterior of the dorsal fin) on <br />the dorsal surface of the fish. Before transmitter <br />attachment, each fis <br />methanesulfonate <br />were placed in a s, <br />33 cm), and the gil <br />solution of MS-22, <br />the keel of each fis <br />0.23-cm bit. The d <br />approximately 3.5 <br />ment suture (Ethic( <br />gauge,needle until i <br />end. The transmitte <br />(two anterior and <br />tightly to the keel. <br />wound following ; <br />the fish was placed <br />approximately 10 <br />spawning site. <br />Data-logging stagy <br />was operated on an <br />area (Figure 1). The <br />antennas with four <br />faced upstream, the <br />the third pointed dii <br />tennas were connec <br />logging receiver. A <br />a photovoltaic pane. <br />tionary logger reco <br />6 June 2000. The re <br />transmitter frequent <br />tagged fish was det <br />cy, code, time, and <br />Signal strength all <br />tional movement. F <br />to determine when r <br />approached and de <br />well as the period e <br />area. For the purpc <br />nocturnal use of tl <br />hours were (onside <br />0659 were consider <br />Ground survey to <br />cific locations of rac <br />bar by monitoring i <br />h periods in May: 1 <br />25. Hourly location, <br />determined (by tria <br />riod. A single Lotel <br />element Yagi antem <br />two of five local be <br />estimated by triang <br />mark locations. Be <br />global positioning s <br />the island adjacent i <br />were measured usii