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For this reason, future brood eggs that have been <br />set aside from paired coatings should be incubated <br />- separately based on the date the eggs were taken. <br />Such a protocol allows adjustments in egg numbers to <br />be made at a later date, insuring that the <br />.contribution from a given spawning take is relative <br />to the number of females actually spawned on that <br />date. At certain facilities, eggs from early takes <br />may actually begin to hatch before the entire <br />population has been spawned. In these situations, <br />"best guess" extrapolations based. on hatchery <br />records should be used to estimate the relative egg <br />contribution"from a given egg take. <br />For Eusmple: <br />Spawn Date -May 1 May 7 May 14 May 21 May 28 <br />Females Ripe 50 250 400_ 250 50 <br />Females used <br />for future <br />brood eggs 25 50 100 50 25 <br />t©tal number should be a minimum of 250 <br />Total number of females ripe on the days brood eggs were taken <br />1000 (50+250+-400+250+50) <br />May 1 = 50 females or 5% <br />May 7 = 250 females of 25% <br />May 14 400 females or 40% <br />May 21 = 250 females or 25% <br />May 28 = 50 females or 5% <br />If 5000 eggs are needed for future brood 5% would come from the <br />paired coatings on May 1(250), 25% from the coatings on May 7 <br />(1250) , 40% from the coatings on May 14 (2000) , 25% from the <br />coatings on May 21 (1250) , and 5% would come from the paired <br />coatings on May 28 (250). This regimen will help to insure <br />genetic variation while maintaining time of spawning. <br />1-6 <br />