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r <br /> <br />V. Laguna Bustillos Basin <br />(A) Arroyo de Nopabechic (Fig. 1, ~~13), 106° 58' N long., 28° 30' W lat., <br />ca. 2140 m, 27 May 1979. -Several Large isolated, stagnant pools, choked with <br />algae and garbage, were seen in the arroyo west of the bridge. Another pool <br />at the base of the bridge (ca. 7 m long, 1 m wide and up to 0.5 rn deep) <br />contained Gila nigrescens. The pool was supplied by a rheocrene with a very <br />slight flow to the east. Sand, coarse gravel, rocks and cobbles formed the <br />substrate, and filamentous green algae were present in the water. Five adult <br />chubs were seen, two adults (ca. 200 mm and 150 mm SL) in breeding color were <br />seined, photographed, and released. Thirty juveniles (less than 40 mm) were <br />captured and five were preserved for study. The total population size in this <br />pool was estimated to be 10 adults and 100 juveniles. The only other species <br />present was Cyrpinodon sp. The Chihuahua chub was last collected here in 1975 <br />(UANL uncatalogued). <br />(B) Arroyo Ojo Caliente (Fig. 1, 4614), 106° 58' N long., 28° 34' W lat., <br />ca. 1990 m, 27 May 1979. -Shallow, flowing water and some small standing pools <br />(ca. 10 cm deep)-were seen.. Within this 30-m wide arroyo, the water was often <br />restricted to 0.5 m or less. Upstream from the bridge the water was clear, <br />without filamentous algae; however, at the bridge a thick surface scum was <br />present. Only pupfish, Cyprinodon sp., and Bufo tadpoles were sighted within <br />200 m above the bridge; no collection was taken. The arroyo was dry below <br />(east of) the bridge. <br />(C) Arroyo Miguel Chiquito (Fig. 1, 4615), 106° 58' N long., 28° 32' W <br />__ lat., ca. 2000 m, 27 May 1979 -This arroyo was totally dry. Gila nigrescens <br />was previously collected here in 1975 (UANL 2493, 2 specimens). <br />(D) Arroyo San Antonio (Fig. 1, 4616), 106° 47' N long., 28° 30' W lat., <br />ca. 1990 m, 27 May 1979. -This large arroyo (ca. 30 m wide) had sufficient <br />running water and pools to support fish. However, no fish or aquatic animals <br />were sighted or encountered. The water was murky green, often with suds and <br />scum floating on the surface. Bubbles were constantly rising from the bottom -~ <br />probably the result of nutrient overload from the surrounding agricultural <br />fields. Thirty-eight specimens of Gila nigrescens were collected here in <br />1964 (UANL 552). <br />Discussion <br />The summary of collection data (Table 1) shows that Gila nigrescens <br />was present at only eight of the sixteen localities surveyed. Furthermore, <br />the chub could be considered abundant at only four of these stations. Two <br />places where the species- was abundant (Rio Piedras Verdes at Colonia Hernandez <br />and Arroyo del Aguila) should perhaps be considered as one locality due to their <br />proximity, thus reducing that number to three. 1'Iiese findings clearly support <br />the listing of Gila nigrescens in New Mexico and Chihuahua as endangered <br />(Hubbard et al., 1978:E-17; Deacon et al., 1979). <br />The latter authors cited (1) present or threatened destruction, modification, <br />or curtailment of habitat or range and (2) hybridization, effect of exotics and <br />4 <br /> <br />