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Anita M. Martinez 5 <br />average total gas pressure, 102.08 °lo). Well water passed through packed columns and <br />then an empty trough (to allow deposition of sediments) prior to entering the test troughs <br />and drained via a surface standpipe. Water temperatures were difficult #o maintain, but <br />fluctuated closely around 20°C, the recommended optimum reproductive and hatching <br />temperature (Hamman 1985; Marsh 1985). Eggs held in flowing water at an average <br />21.1°C began hatching 96h postfertilization and all fry had emerged after 129h. Hatching <br />trays were removed following completion of hatching and larvae were maintained in the <br />rearing trays. <br />Swim up by larvae began on the fourth day post hatching and swim up was <br />completed on the seventh .day. On the ninth day posthatching, three subsets of 200 larvae <br />from each cross were placed in separate rearing trays in the same trough. All siblings <br />not included in the three subsets were placed in a fourth tray located at the outlet end of <br />the trough. fn this way, integrity of all four parental crosses was maintained. One, of <br />three, test diet was fed to each subset in each of the four troughs for 37days {until larvae <br />were 46d old). The diets included B-250 (Fry Feed Kyowa 6-250; BioKyowa, Inc., <br />Chesterfield, Missouri), HEG1 (Hatchfry Encapsulon Grade 1), and HEG1+ (HEG1 mixed <br />with Spirulina Regular in a 9:1 ratio by weight Argent Chemical Laboratories, Redmond, <br />Washington). All diets were subjected to proximate analysis (AOAC 1984). Siblings not <br />included in the subsets were fed B-250 ad lib five times daily. Larvae in each subset were <br />also hand fed a total ration of 10% of their body weight five times daily. <br />Rations were calculated from the initial average larvae weight of 0.005 g reported <br />