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The Gila complex (bonytail, humpback chub, and roundtail chub) in <br />the Upper Colorado River Basin has posed some concern about <br />potential hybridization based on the results of the morphometric <br />analyses by Michael Douglas, Arizona State University and allozyme <br />analyses by Wayne Starnes, Smithsonian Institution. Although <br />hybridization between the three species was possible in a hatchery <br />(Hamman 1981), it is not known if these species will spawn together <br />in the wild. Since all three species overlap temporally during <br />their spawning seasons, one recommendation was to conduct a <br />laboratory or controlled field experiment to determine if spatial <br />or behavioral isolation exists between the Gila species. The adult <br />humpback chub from Black Rocks could be used in this experiment. <br />Another recommendation was to determine if F, fish produce viable <br />gametes and if these hybrids may be indiscriminate in spawning <br />behavior. Adult fish in the refuge could be spawned to produce F, <br />hybrids and these progeny could-be spawned to determine the <br />viability of their gametes and potential to produce F2 hybrids. No <br />mortality should be encountered through use of the wild fish in <br />either study. <br />E. Humpback Chub - Westwater Canyon. The collection of adult humpback <br />chubs from Westwater Canyon will be postponed since there is no <br />genetic data to indicate that the two stocks are separate. Some <br />exchange between the two locations has been documented. Three <br />adult humpback chubs of 43 tagged in Westwater Canyon moved <br />upstream to Black Rocks Canyon (Valdez et al. 1982). About 1,200 <br />humpback chubs were PIT-tagged by the Utah Division of Wildlife <br />Resources through 1994 and most recaptures of these fish were close <br />to the point of release (T. Chart, 1994, Personal Communication). <br />Several of the fish tagged by Utah in Westwater Canyon were <br />recaptured by the Colorado Division of Wildlife in Black Rocks <br />Canyon (B. Elmblad, 1995, Personal Communication). <br />F. Long-Term Research. Razorback suckers and Colorado squawfish are <br />being reared at the Ouray Endangered Fish Facility to determine if <br />these fish species imprint to spawning areas from olfaction cues <br />(Scholz et al. 1993) based on the strong fidelity to spawning areas <br />by Colorado squawfish (Tyus 1990) and razorback suckers (Tyus and <br />Karp 1990). <br />Some of the male razorback suckers will attain a total length of <br />400-425 mm (about 16-17 inches) and over 60 were mature in the <br />spring of 1996. These fish will be tested during 1996 for <br />preliminary information related to imprinting. Most of the <br />razorback suckers are expected to mature (at 17 inches TL) in the <br />spring of 1997 when results should be obtained to make a <br />comprehensive evaluation as to whether or not razorback suckers <br />imprint through olfaction cues. <br />Improved growth of the chemoreception study fish is expected in <br />1996 when they will be reared at much lower densities in the new <br />8