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i r <br />males have long anal fins (when compressed the anal fin will <br />extend beyond the origin of the caudal fin) while females <br />have shorter anal fins that do not extend beyond the origin <br />of the caudal fin. These relationships are not absolute but <br />are valid for a large proportion of the specimens (e.g., <br />85-90% in some studies). Mass marking of small fish has been <br />done in a variety of ways with varying results. These <br />marking techniques should be reviewed to select some marks <br />for testing the effects on the fish, retention, and recovery. <br />The marking of small fish would allow investigators to <br />determine the extent (speed and distance) of movement and add <br />to our understanding of the significance of this dispersal <br />mechanism. For example, several of the confirmed spawning <br />areas for Colorado squawfish may be contributing the majority <br />of the young for the system and, therefore, may be highly <br />critical in keeping the species from becoming extinct. <br />o Obtain essential information on the <br />the Colorado squawfish, other rare <br />that are common in the upper basin. <br />biology and habitat requirements for <br />species, and selected endemic species <br />- Determine the extent and significance of downstream larval movement <br />(both passive drift of early larvae and active movement of advanced <br />larvae) of the Colorado squawfjsh as a potential mechanism for <br />populating suitable nursery areas. <br />- Examine the availability and size of food organisms (plankton and <br />benthos) in the shallow backwater habitats of the upper basin to <br />determine if the quantity (abundance) and quality (size) of potential <br />food organisms is a limiting factor in survival of young rare fish. <br />Conduct studies under controlled conditions (e.g., hatchery or <br />laboratory) to determine food preference of young rare fish. <br />- Review the systematics of the Gila sp. through coordinated, concurrent <br />studies on the same specimens. <br />Rationale - Gaps exist in the knowledge of the biology and <br />habitat requirements of the Colorado squawfish. For example, <br />habitat utilization curves for adult squawfish have only been <br />developed for spawning. In addition, the extent and <br />significance of downstream larval movement needs further <br />study since it may be critical to the survival of the <br />species. Some life history and habitat requirement <br />information has been collected on the razorback sucker but we <br />still do not have adequate information to provide sound <br />biological opinions regarding this rare species. Very little <br />information is known about the biology and habitat <br />requirements of the humpback and bonytail chubs. The <br />bonytail chub is on the verge of extinction, the humpback <br />chub is found primarily in two major areas of the UCRB, and <br />the razorback sucker is rare. Mortality of the young rare <br />8