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12 FRED P. MEYER AND ROGER L. HERMAN <br />See Chapter 7 for instructions on what additional <br />data are needed and how the information should <br />be documented. An analysis of this information <br />often makes it possible to rule out several potential <br />causes of a fish kill and may make it possible to <br />distinguish between one or two likely or suspected <br />causes. This reduces the number and types of sam- <br />ples that are required and helps reduce the person- <br />nel, equipment, and laboratory work needed when <br />time is critical. <br />In recording fish kills, it is important to establish <br />the magnitude of the mortality. The significance of <br />a fish kill is always directly related to economic, <br />geographical, and political factors associated with <br />the site, as well as to the ecological effects. The <br />losses of 100 fish in a prime trout stream or any <br />losses due to a possible toxic discharge are always <br />important; in other situations, the loss of thousands <br />of gizzard shad may be of little public concern. The <br />American Public Health Association (APHA) et al. <br />(1985) offers the following guide for reporting fish <br />kills: <br />Minor kill: less than 100 fish <br />Moderate kill: 100 to 1,000 fish in 16 km of stream <br />or equivalent lentic area <br />Major kill: more than 1,000 fish in 1.6 km of a <br />stream or equivalent lentic area <br />The rate or pattern of loss is a helpful indicator <br />(Fig. 3.1). If all fish died abruptly or within a short <br />time (24 hours or less), it is likely that the kill was <br />caused by a sudden, catastrophic event that made <br />the environment fatally toxic to fish. If the mortal- <br />ity began slowly and then rose sharply over the next <br />5 to 7 days, the most likely causes would be a slow- <br />ly developing oxygen depletion or a highly virulent <br />infectious agent. Mortality that continues at a low <br />rate over an extended period may be due to a mar- <br />ginal environment, a low-virulence infective agent, <br />or chronic exposure to sublethal concentrations of <br />a toxic substance. <br />A second important piece of information is that <br />of the sizes and species of fish affected (Table 3.1). <br />In kills caused by toxic substances, small fish usual- <br />ly die before larger ones of the same species; in <br />oxygen depletion, the reverse is true. <br />Establishing when a kill began and how long it <br />continued is also often important. It is useful to <br />know whether the kill began at night, how long it <br />continued, and whether it was interrupted for a time <br />and then began anew. <br />100 <br /> 1 <br />Oxygen depletion <br />l <br />th <br />l <br />i <br /> 1 or <br />e <br />a <br />tox <br />cant <br /> <br /> highly <br />t 1 <br />1 virulent bacteria <br />O 1 or virus infection <br />? i <br />O <br />D 1 <br />a? 1 <br />1 <br />C <br /> <br /> <br />U external parasites, <br /> 1 low-virulence bacteria, <br /> I poor environmental <br /> conditions, or <br /> <br />1 chronic exposure <br /> to pollution <br /> 1 2 3 4 5 6 7 8 9 10 11 <br /> Time (days) <br />Fig. 3.1. Curves (mortality <br />versus time) associated <br />with three major categor- <br />ies of fish mortality (Wede- <br />meyer et al. 1976).