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-50- had large rust spots on them. In Basket Na. 1, 41.970 of the dead eggs showed rust spots, and 9.27. of the dead eggs in Basket No. 2 exhibited this condition. This large amount of dead eggs had served for the develdpment of fungus which enveloped many sur- rounding eggs. Following the above experiments, inquiries were made to other states concerning the effect of low temperatures on the viability of kokanee eggs. A copy of a letter sent to the .Colorado Game and Fish Department by Mr. Roger Burrows, Chief of the Salmon-Cultural Laboratory in Entiat, Washington, was received. Mr. Burrows stated that experiments conducted with sockeye (Oncorhynchus nerka) eggs indicated that below 42.5" F. losses were abnormally high. At 35® F. the mortality was in excess of 80~. Also, the work indicated that low temperatures caused mortal-it'y ohly during early stages of develop- ment. Once the eggs had passed the 128 celled stage of development, they could stand temperatures of 35° F. with impunity. Information from the State of Montana stated that eggs were held in tem- peratures from 46® F. in November to about 38® F. in January with a good per- centage of survival and hatching. The days at which .the eggs were incubated before being subjected to low temperatures, if any, were not given. It was decided from the results of inquiries that additional experiments should be conducted with green eggs to determine eyeing and. hatching success. During this segment additional groups of eggs we're planted. Pine Creek, at the inlet of Freanont bake, was used to determine hatching success to be expected from fingerling plants made there to establish spawning runs, and 40 Rod Creek adjacent to the .Daniel Hatchery was used to compare eyeing-and hatching success in waters having temperatures above 43® F. Eggs were also placed iu*n the Daniel Hatchery ine~abator as a control. The planting procedure used was the same as that described for previous plants using eyed kokanee and brown trout eggs in 1958 and 1959. The baskets were painted with a rust retarding hatchery trough paint. The steps followed, from stripping of the eggs to planting in the selec- ted sites, have been listed belowo December 2, 1960 (1) Eggs stripped at Granby Reservoir in Colorado, packed in quart jars and placed in panniers. Sawdust was used as insulation. Time at completion of this operation was 11x30 a.m. (2) Eggs picked up by Wyoming Game and Fish Department airplane at 1e 30 p.m. (3) Eggs delivered at Pi~aedale, Wyoming at 4000 p.m., temperature on arrival was 39° F. (4) Eggs were hauled to the Daniel Hatchery, approximately 14 miles, tempered to hatchery water anal measured into 18 quart jars. Three ounces of eggs were placed in each jar. This procedure was taken to expedite planting