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,:. effects of fluctuating flows on Cladophora life history; 4) <br />determine the effects of fluctuating flows on phytoplankton and <br />zooplankton life stages and the utilization and importance of <br />these plankton to the fish species at various life stages <br />throughout the study area; and 5) determine the importance of <br />natural reproduction versus artificial propagation to the <br />maintenance of the trout fishery in the Glen Canyon tailwater. <br />In addition to these objectives, we were to include, an analysis <br />of potential alternative operating criteria consistent with <br />Colorado River Storage Project (CRSP) requirements and to propose <br />feasible alternatives. <br />The first objective was to collect baseline data on fish <br />species composition and distribution in the Colorado River. This <br />information allowed the more important second objective of <br />comparing use of mainchannel and tributaries for spawning and <br />nursery areas for larval fishes and rearing of older fish. <br />Objective three was addressed through a combination of field and <br />laboratory studies by researchers at Northern Arizona <br />University. Because larval life stages of fishes may be <br />dependent on zooplankton, objective four was included. A <br />combination of plankton samples and larval fish gut analysis was <br />incorporated to address this objective., The fifth objective was <br />to establish whether rainbow trout in the Glen Canyon tailwater <br />were naturally reproducing and, if so, how much they contributed <br />to the creel. Other aspects of the ecosystem not covered in <br />these objectives but considered important were also addressed. <br />The five discharge scenarios are based on the minimum <br />release (8..3 million acre feet [maf]) established by law that <br />must be released annually through Glen Canyon Dam. A release <br />pattern based on 8.3 maf could have almost any combination of <br />steady and fluctuating flows. A major factor that affected <br />collection and analysis of data was the amount of runoff during <br />the study period. Annual releases from the dam ranged from 16.6- <br />21.1 maf during our study. Because of these high releases BOR <br />was not able to provide the various flow patterns necessary to <br />address all the questions raised in the study objectives. Most <br />data collection occurred during periods of high steady <br />releases. Only brief periods of fluctuations were provided <br />during the study, and these generally came during autumn and <br />winter. <br />4 -12-