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3 IDENTIFICATION OF HABITAT REQUIREMENTS AND LIMITING FACTORS FOR COLORADO SQUAWFISH AND HUMPBACK CHUBS Charles M. Haynes and Robert T. Muth PROGRAM NARRATIVE OBJECTIVE The overall objective of this investigation is to determine the physical/ biotic factors which limit the reproduction and distribution of Colorado squawfish (Ptvchochielus lucius) and humpback chubs (Gila cypha) in Colorado. Similarly, this project is designed to develop field/laboratory methods for evaluating squawfish and humpback chub habitat and reproductive success which may be employed by management personnel for future habitat enhancement and/or reintroduction programs in accordance with overall recovery efforts. Information derived from this study will be made available to management and research personnel in the Division of Wildlife as well as other cooperating agencies through annual reports, presentations, and technical manuscripts. Additionally, aspects of this project relating to the humpback chub will be incorporated into a doctoral dissertation in 1984-85 by the junior author. SEGMENT OBJECTIVES 1. To identify and describe quantitatively those habitat parameters which limit the distribution and abundance of. Colorado squawfish and humpback chubs in the Upper Colorado River, with particular reference to larval and juvenile stages. 2. To develop methods for evaluating larval/juvenile squawfish and humpback chub habitat and reproductive success which may be employed by management personnel for future habitat enhancement and/or reintroduction programs. 3. To develop methods for the identification of larval and juvenile humpback chubs and differentiation of humpback chubs from immature stages of the roundtail chub and possible Gila spp. hybrids and/or intergrades. 4. To develop baseline criteria for both water quantity and quality as a tool for predicting and mitigating potential impacts from future river development. 5. Compile data and submit reports to appropriate state and federal offices. Note - These objectives are in accordance with tasks 111 and 1111 of the approved Colorado squawfish recovery plan (1978) and tasks 11, 111, 41, 42, and 43 of the approved humpback chub recovery plan (1979).