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7/14/2009 5:02:32 PM
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UCREFRP
UCREFRP Catalog Number
7970
Author
Dowling, T. E. and W. L. Minckley.
Title
Genetic Diversity Of Razorback Sucker As Determined By Restriction Endonuclease Analysis Of Mitochondrial DNA.
USFW Year
1994.
USFW - Doc Type
Bureau of Reclamation, # 0-FC-40-09530-004,
Copyright Material
NO
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through 1988. DNFH records indicate most of the Fl broodfish were derived from six to <br />eight 81-WILD females (two of which contributed 95.6% of the 29,815 larvae [hereafter <br />81 YC-Ft] produced that year). <br />Seventy-one wild females (derived primarily from 1982 collections but also including a <br />few surviving 1981 wild fish) were spawned in 1982 to produce 1.7 million larvae (hereafter <br />82YC-F1). There is no specific record for any of the 82YC-Fts being kept as broodfish, but <br />two lines of evidence make it clear that some were retained. First, variation of mtDNA for <br />later year classes cannot otherwise be explained. The high diversity (h=0.89) and number of <br />haplotypes per individual (0.60) in the 1987 progeny (87YC-F2s) aze evidence that the <br />females used must have included a substantial proportion of 82YC-Fts (see Appendix II). <br />The small number of females producing 81YC-Fts almost certainly resulted in a reduction in <br />diversity and must have dramatically reduced haplotype number in that year class, which <br />would have been exacerbated in production. of 87YC-F2s. Second, reference in the DNFH <br />Annual report for 1986 to "4- and 5-yeaz-olds" must include broodfish from both 81YC-Ft <br />and 82YC-Fl. We are therefore certain that Fls were retained as broodfish both in 1981 and <br />1982, likely more individuals from the latter, and contributed to later DNFH production (see <br />Appendix In. <br />In addirion to 81+82YC-Fls, wild fish captured as larvae in 1985 and reazed in captivity <br />(85YC-WILD) may also have contributed to production in 1989, continuing into later year <br />classes. Thus, the 14 females spawned in 1989 may have been derived from 81+82YC-Fts <br />and 85YC-WILD. The resulting progeny exhibited h and haplotype numbers similar to that <br />of 1987 (Table 4). <br />In 1990, the 526 fish in the broodstock were derived from 81+82YC-Fls (likely more <br />than 50%), 85YC-WILD (likely less than 20%), and 176 (33.5%) 87YC-F2s. Seventeen <br />22 <br />
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