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suckers at DNFH was obtained in 1981-82 from Lake Mohave, a mainstem impoundment on <br />the Colorado River, Arizona-Nevada, created in 1954 by closure of Davis Dam. The <br />reservoir consists of an upstream riverine reach approximately 60 km in length, which grades <br />into a wider (maximum width 6.4 km) lower basin 48 km long. It has a surface area of 115 <br />km2, average depth of 10.5 m (maximum approximately 43 m), and a volume of 2.3 x 109 m3 <br />at full pool (197 m above MSL). Retention time is short, with total replacement taking only <br />0.24 year at an average annual discharge of 9.3 x 109 m3. <br />Tag-recapture data indicate a population of less than 55,000 adult razorback suckers in <br />the reservoir (Marsh, 1994). Although scattered throughout, the fish are concentrated in upper <br />parts of the lower basin. They are most vulnerable to capture when aggregated near shore for <br />spawning in winter-spring, but not as readily collected when dispersed at other times. The <br />species has made up 10-30% of net catches in spawning areas over the past 20 years <br />(Hinckley, 1983; Hinckley et al., 1991). <br />Production of hatchery year classes.- Development of procedures to culture razorback <br />suckers began with Toney (1974), were refined by Inslee (1982) and Hamman (1985}, and <br />soon became routine (Hinckley et al., 1991). Adult fish are held in outdoor ponds and <br />transferred to indoor holding facilities in spring for spawning. Ovulation is induced by <br />intramuscular injection of human chorionic gonadotropin (up to three 220 IIJ per kg injections <br />at 24-hour intervals). When necessary to induce or maintain milt production, males are <br />injected with 660 IU common carp (Cyprinus carpio) pituitary extract. <br />Ova are hand-stripped into water, sperm from two or three males is added, and the <br />nnixture gently stirred. After addition of bentonite to counteract adhesion, fertilized ova are <br />rinsed in fresh water and allowed to harden for about 0:75 hour in floating Nitex®-mesh <br />baskets. Developing embryos are transferred to flow-through (18-22°C), HeathC~incubator <br />17 <br />