2004

• UCREFRP Catalog Number: 2004
• Author: MacPhee, C. and M. H. Beleau.
• Title: Acute Toxicity of Selected Chemicals to the Colorado Squawfish
• USFW Year: 1980.
• USFW - Doc Type: An Endangered Species.
• Copyright Material: NO

.~
'
' r
'w ..
•
•
The humpback chub (Gila ~~) is not presently being addressed in
these studies. If sufficient numbers of fish become available in the
future, toxicity studies with this species can be incorporated at ti~at
time.
3
Water
Thrae different waters were originally propo fed as candidate fluids
for use in the bioassay studies: Palouse River water, artesian well water,
University of Idaho, and simulated Willow Beach. water, Arizona. The volcanic
eruption . of Mt. St. Helens, Washington, coated the Palouse River watershed
with ash. This and the need for standardization of the bioassay procedures
made the use of Palouse River water undesirable to simulate Willow Beach
water. To date, two formulations of water have been utilized (Table 1).
The first of these formulations utilized distilled water as a base and
sufficient chemicals were added to duplicate an earlier chemical analysis
of Willow Beach water (designated SWB). The large amount: of distilled water
required and cost of chemicals and labor involved, made it necessary to derive
a second formulation utilizing artesian: well tap water (designated TSWB} as
~. a base. Chemicals added to the well water permitted a close and adequate
' approximation of the Willow Beach analysis which itself varies with time
and distance from other site locations on the Colorado River. All of the
bioassays conducted to date utilized either SI~TB or TSWB water.
Facilities
~ ~ Facilities included tanks for holding and acclimating test organisms and
for conducting bioassays. Constant temperature-rooms and recirculating water
i
baths maintain water temperatures. Oil and fume free compressors are used
to ~to~ida sit for aeration. Twa laboratory-areas at the University of Idaho