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Science and technology form the foundation of successful fish and aquatic resource conservation and are used to structure and <br />implement monitoring and evaluation programs that are critical to determine the success of management actions. The Service is <br />committed to following established principles of sound science. <br />AZFRO Completes Study to Determine the Effects of Repeated Fish Handling <br />AZFRO is responsible for monitoring endangered <br />humpback chub populations within the Little <br />Colorado River, AZ, near Grand Canyon National <br />Park. Current and past monitoring and research <br />activities often include initially capturing and tagging <br />fish and then subsequently recapturing the same fish <br />numerous times to generate population estimates. In <br />FY 2004, AZFRO completed a study that assessed the <br />effects of repeated handling on growth, condition, and <br />survival of humpback chub, using bonytail as a <br />surrogate. Final results indicate that fish that were <br />repeatedly handled weighed less than fish not <br />handled; however, there was no difference in length. <br />Handling fish is inevitable and essential to <br />understanding population dynamics; however, based <br />on our findings, whenever possible, researchers <br />should make every effort to minimize the effects of <br />sampling on fish. <br />AZFRO Continues Watershed Prioritization for Future Conservation Efforts <br />I n cooperation with Federal, state, and private agencies, AZFRO developed a watershed index of biotic integrity (IBI) <br />for identifying watershed with conservation potential and ranked each watershed based on native species diversity <br />and current distribution. Using the IBI scores we were able to determine which physical variables (i.e., number of <br />dams, impounded stream miles, groundwater pumping, etc.) affect watershed scores. AZFRO hopes to develop <br />strategies for watershed-level restoration based on level of community watershed activity and conducive land <br />ownership. Information from this effort was used to develop a Region 2 Habitat Initiative. <br />AZFRO Biologists Publish Research Findings <br />I n FY 2004, AZFRO biologists have had results from projects conducted over the past several years accepted for <br />publication in peer-reviewed journals. Titles of manuscripts accepted for publication include `Age, growth, and <br />reproduction of roundtail chub from the headwaters of the upper Verde River" and "Differential detection of ingested <br />items evacuated from genus Gila cyprinids by two non-lethal alimentary tract lavage techniques." An additional <br />manuscript that was submitted and is being revised for publication in FY 2004 is titled `Effect of baiting on hoop net <br />catch rates of humpback chub. " <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br />8 Arizona Fishery Resources Office FY 2004 Annual Report ' <br />