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The number of samples taken is a function of the variance within the <br />population to be sampled. If explicit zonation is used to generate the <br />population, then only one sample from each population is needed, although <br />more might be taken to satisfy any uncertainties concerning the zonation. <br />If implicit zonation is used, at least three samples should be taken. The <br />purpose of this exercise is to determine areas which are to be inspected <br />on-site, without having to inspect the entire river. Therefore, the <br />number of samples taken is not critical, but it should not be more than <br />about half the total population of candidate reaches. <br />Now that a small number of candidate reaches have been selected, the <br />investigator should arrange for an on-site inspection of each selected <br />"representative reach." If, on inspection, all of the representative <br />reaches are fairly similar, only one is required as a location for a study <br />site. In this case, such considerations as access, logistics, and <br />landowner permission may guide the selection of the study site. However, <br />if on-site inspection reveals that all of the "representative reaches" are <br />significantly different, it may be necessary to either set up study sites <br />in each reach or revert to an explicit zonation and resample the <br />population of candidate reaches. <br />At this time, it is necessary to determine the length of river within <br />the stratified segment represented by each selected candidate reach. If <br />explicit zonation is used, the represented reach length equals the river <br />length in each explicitly defined zone. If implicit zonation is used, the. <br />length represented is proportional to the number of samples taken. If <br />four samples are taken, and each is to be studied separately, then each <br />selected representative reach represents 25% of the total stream length <br />within the stratified segment. However, if after inspecting the four <br />sites, three seem to be very similar and one different, then only two <br />selected representative reaches are used, one representing 75% of the <br />segment length and one representing 25%. <br />The study site itself is then set up within the representative reach. <br />The study site may encompass the entire representative reach, or a portion <br />of it. The guiding principle is that the study site should be a sample of <br />most of the variance in habitat types, in the proportion that they occur, <br />within the representative reach. If this variance can be described in <br />only half of the representative reach, then the study area may encompass <br />this area only- <br />ESTABLISHMENT OF STUDY AREAS <br />As previously mentioned, a study area may encompass the entirety of <br />the selected representative reach, or only a portion of it, depending on <br />the variation in habitat encountered within the representative reach. The <br />purpose of the study area is to provide a sample of the variation in <br />hydraulic conditions and habitat types, as well as the proportion occupied <br />by each habitat type within the representative reach. The variation and <br />proportionality of habitat types are determined through the use of <br />strategically placed transects, delineating discrete areas of the stream. <br />y8