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After completion of the sampling or observation run, the observer <br />returns to each buoy, where measurements are made of the depth, velocity, <br />substrate, and cover. The velocity sought is the mean velocity in the <br />water column, therefore, velocities should be measured at 0.6 of the <br />depth (from the surface) if the water is less than 3 feet (90 cm) deep. <br />If greater than 3 feet (90 cm), two measurements, at 0.2 and 0.8 of the <br />depth should be averaged. Substrate size should be estimated from the <br />modified Wentworth scale (Table 2). In water deeper-than 4 feet (120 cm), <br />these measurements may be made from a boat using cable sounding gear and <br />a current meter. <br />For univariate frequency analysis, a total of approximately 300 <br />measurements for each life stage of each species are needed to ensure <br />a representative analysis. For multivariate analysis, 1,000 to~2,000 <br />measurements are required. <br />OPTION 2 - AREA GRID MEASUREMENTS <br />This method has its best application with species or life stages <br />which school or form large congregations. Collection is nearly always <br />required because the subjects are often small and difficult to identify <br />by simple observation. Exceptions would include spawning congregations <br />of adults where there is little doubt concerning the species, life <br />stage, and activity. <br />Sampling techniques amenable to this method provided the subjects <br />can be identified and counted, include electrofishing, spot concussion, <br />seining, and observational techniques. Markers are used to outline <br />areas of fish concentrations as determined from sampling or observation. <br />A measurement grid is established visually over the outlined area <br />(Figure 6} and the number of individuals of the species counted for <br />each outlined area. Hydraulic parameters are measured at each grid <br />intersection. Measurements of each parameter are then averaged, and the <br />standard deviation calculated. If the standard deviation is greater <br />than 25% of the mean, the sample should be discarded and option 1 used <br />instead. Under option 2, the mean of several measurements (tested for <br />variance) is used to represent a large number of individuals, rather <br />than one at a time as in option 1. <br />Under the area grid approach a total of 100 to 200 outlined areas <br />should be measured for each life stage and species. <br />23 <br />