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macrohabitat characteristics such as water quality or temperature. Micro- <br />habitat characteristics are measured at other study sites. The basic habitat <br />accounting unit is the river segment, a relatively long reach of stream <br />exhibiting homogeneity in channel characteristics and flow regime. Guidelines <br />for establishing segment boundaries include places where the average base flow <br />changes by 10 to 15%, or where changes in slope, channel dimensions, or channel <br />pattern are apparent. <br />A river segment may contain one or more study sites for macrohabitat <br />and/or microhabitat measurements. A single study site is used to describe one <br />or the other type of habitat, but generally not both. Macrohabitat study <br />sites include control sites, point sources, and a network of stations to <br />define concentration or temperature profiles along the stream. Control sites <br />are used to determine the background concentrations or temperatures above the <br />influence of chemical or thermal inputs. Point sources include outfalls of <br />pollutants, runoff points for aggregated nonpoint sources, and confluences of <br />tributaries. Many biologically important water quality constituents change <br />concentration through chemical and biochemical reactions as they move down- <br />stream. A network of monitoring stations, often evenly spaced along the <br />stream, is used to define such concentration or temperature profiles. <br />Several types of study sites are utilized for the measurement of micro- <br />habitat characteristics. Representative reaches are selected through a random <br />or uniform sampling process, and are used to describe the typical microhabitat <br />in a segment. Several representative reaches may be needed in segments <br />exhibiting gradual longitudinal changes in slope, channel dimensions, or <br />channel pattern. Critical reaches are generally atypical of the microhabitat <br />in a segment. The two criteria used to define a critical reach are: <br />1. The microhabitat characteristics of the critical reach are <br />controlling or limiting to the evaluation species (such as <br />limiting migration or spawning); and <br />2. These microhabitat characteristics are unavailable or in short <br />supply in the representative reaches. <br />A special type of critical reach is termed a unique reach. Unique reaches <br />apply only to endangered species, and are typified by large concentrations of <br />these species in streams where such concentrations are unusual. The reasons <br />for the concentration of an endangered species are usually unknown, but a <br />unique reach can be designated by virtue of the status of the evaluation <br />species. <br />The analytical sequence followed in an application of the IFIM consists <br />of six steps: <br />1. Describing the river or system in its present state; <br />2. Determining the mathematical expressions and functional rela- <br />tionships describing the temporal macro- and microhabitat <br />availability of the present system and integrating to determine <br />total habitat availability; <br />vi