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Extensive testing for the presence of the parasite in wild trout populations all across the <br />state. To date, we have sampled only a small percent of the state's salmonid habitats, <br />but have tested all of the state's major trout fisheries. Our goal is to have statistically <br />valid samples completed on all Colorado streams by 1999. To date, all major trout <br />streams and most large cold- and coolwater impoundments have been tested. Areas <br />still needing testing are largely confined to small streams and remote high mountain <br />lakes. <br />2. Monitoring the level of virulence and infectivity of the disease in most major streams in <br />the state that are currently affected at the population level (loss of year classes), <br />including, but not limited to, the Arkansas, Big Thompson, Blue, Cache la Poudre, <br />Colorado, Dolores, Fryingpan, Gunnison, Rio Grande, Roaring Fork, South Fork of the <br />Rio Grande, South Platte, and Taylor rivers. <br />Susceptibility testing of many strains and species of salmonids to determine their <br />vulnerability to the WD pathogen, including brown trout, brook trout, A+ Colorado <br />River cutthroat trout, Trappers Lake cutthroat trout, Colorado River rainbow trout, <br />and Tasmanian rainbow trout. This effort will be continued in 1996 and will include <br />tests on the Rio Grande and greenback cutthroat trout and the Snake River cutthroat <br />trout, along with other salmonids. <br />4. Annual population-level monitoring as to the status of wild rainbow and brown trout <br />populations in the streams in 42 (above) to assess whether or not there is a change in <br />the effects of WD on wild salmonid populations. <br />5. Collection and testing of Colorado River cutthroat trout populations from 17 streams <br />on the west slope during 1995. Encouragingly, all populations tested negative for WD. <br />This effort is being continued and expanded in 1996. <br />6. Conducting a research project to answer the question of whether the continued <br />stocking of trout from WD+ units into WD+ habitats increases or maintains WD spore <br />levels above that produced by the alternative tubifex worm host. <br />7. Collection and preservation of aquatic oligochaete worms from the same streams where <br />Colorado River cutthroat trout were captured and tested in 1995 to determine if the <br />alternate host of the pathogen occurs in cutthroat trout habitats. <br />We recommend that DOW biologists cooperate and share knowledge with other scientists <br />and agencies involved in WD research. If this is done, everyone will benefit. As the magazine <br />Fisheries recently noted, "A coordinated approach to investigating whirling disease across the <br />country would shorten the learning curve, thereby benefitting public resource stewards and the <br />private sector. It is time to search for answers and knowledge, not scapegoats." (Hulbert 1996). <br />9