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2. Limnology <br />Once five study sites are selected, a more thorough analysis of <br />biotic and abioti-- features will be conducted: The basic limnologi- <br />cal data outlined above will be collected at a minimum of once per <br />month for the duration of the study. Temperature and DO readings will <br />be taken at the surface, mid-depth, and bottom. DO will be measured with <br />a Hach Model OX-2P DO test kit; temperature and conductivity with a <br />Yellow Springs Model 33 S-C-T meter; and transparency with a standard <br />21 cm dia. secchi disc. <br />3• Fish Community Composition <br />Population estimates of largemouth bass present in each pond will <br />be made using the Peterson-Lincoln mark-recapture estimator as modi <br />fied by Chapman (1951). Electrofishing, seining, and trammel-netting <br />will be used to capture bass for the population estimates. Captured <br />fish will be marked with a pelvic fin-clip, measured for Total Length, <br />and then released. Because the Peterson-Lincoln estimator does not <br />require constant effort, data gathered with the different gear types <br />will be combined. Separate estimates will be made for three size- <br />classes of bass; small (90-160 mm TL), medium (161-280 mm TL), and <br />large (281-450 mm TL). <br />Seines will be of two different sizes; one 30.5 m long x 1.8 m deep <br />and the other 137 m long x 2,4 m deep, both with 6 mm mesh. Trammel <br />nets will be of 2.5 cm bar mesh with a 25.4 cm mesh outer wall and <br />45.0 m long x 1.8 m deep. Electrofishing will employ a 4.9 m boom-shock- <br />ing boat equipped with a Coffelt WP-25 unit and 4.0 kilowatt, 220-volt <br />generator. <br />Relative abundance of forage-sized fish (C70 mm long) will be deter- <br />mined using seining. The seine.used will be the smaller of the two <br />described above. The number of seine hauls per pond during each seining <br />effort will be based on one haul per 61 m of shoreline. A minimum of <br />two seining efforts will be made at each pond each summer. Individuals. <br />of each species will be counted. The lengths of 20 randomly selected <br />individuals of each species will be measured in each of five randomly <br />selected seine hauls at each pond for length frequency analysis. <br />4. Squawfish Stocking <br />Colorado squawfish 40-145 mm long (TL) will be stocked at a rate of <br />3090 per hectare in each of the ponds a total of three times. The first <br />32