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test their response periodically until mature. One group of 500 <br />fry will be exposed to morpholine from hatch to one month of age, <br />approximately the time spent in the Yampa River by wild, recently <br />hatched fry (Archer and Tyus 1984). Another group will be <br />treated similarly, but not exposed to morpholine, to serve as a <br />control. Morpholine is a heterocyclic amine that does not occur <br />in natural waters , is infinitely water-soluble, relative stable, <br />and is perceived at low thresholds by salmon (Wisby 1952). Fish <br />will be tested for morpholine recognition after growth to a <br />sufficient size for implanting EEG electrodes (probably at 18 <br />months of age) and every 6 months therafter until mature. The <br />responses of the imprinted and nonimprinted specimens (12 fish <br />for each group) will be compared using a Mann-Whitney U test. <br />Squawfish mature at 5 to 7 years of age, a captive fish held <br />in optimum growing conditions might reach maturity at 3 years of <br />age (J. Valentine, Fishery Biologist, U. S. Fish and wildlife <br />Service, Salt Lake City, Utah, Personnal communication). <br />Imprinted fish need not be mature to recognize the imprinting <br />chemical (Dizon et al. 197.5), although mature, ripe fish elicit a <br />more pronounced response (Nara and Brown 1979). <br />EXPECTED RESULTS <br />Results from these tests will provide the best information <br />possible from available stocks of Colorado squawfish on ability <br />of the species to identify specific water by sense of smell and <br />presumably then to return to home waters in the wild. In <br />addition, information will be gained on the influence of age and <br />sexual maturity on olfactory recognition of home waters. <br />4