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METHODS <br />Size specific sampling techniques were employed for larval and adult individuals. <br />Habitats sampled for individual species differed spatially and temporally, but not representatively <br />at the selected life stages. Habitats sampled for adult fishes consisted of main channel <br />shorelines, flooded tributaries, mouths of ephemeral washes, and main channel cobble bars. <br />Sampling for razorback sucker adults was conducted on the ascending limb of the hydrograph in <br />April-May of 1994 and in May-June of 1995. Colorado pikeminnow adult sampling occurred in <br />late June in both 1994 and 1995. Razorback sucker adults were only sampled using <br />electrofishing. Sampling for razorback sucker adults consisted of three electrofishing trips in <br />1994 and four trips in 1995. In 1994, trammel nets were used to sample for adult Colorado <br />pikeminnow in the vicinity of Millard Canyon. In 1995, pikeminnow adults were sampled with <br />both trammel nets and electrofishing (refer to Appendix table 1 for a summary of sampling <br />efforts for all life stages) <br />Electrofishing was conducted from a 4.8-m long aluminum john boat with AC power <br />supplied from a 5 KW generator and converted to pulsed DC by a Coffelt Variable Voltage <br />Pulsator (WP-15). Metered readings of main channel shocking values were 300-500 V, 5-7 A, <br />and 20-40 Hz depending on water conditions. Sampling of flooded tributaries and ephemeral <br />washes was hampered by high water conductivities (>1500 ,uS). <br />Trammel nets used were 23 m X 1.8 m with inner/outer mesh sizes of 2.5/25 cm, <br />respectively. Nets were sampled either by drifting them through runs or by securing one end to <br />shore. Passive set trammel nets were checked for fish every 2 hours and were set in place for up <br />to 5 hours. All fish captured via electrofishing and trammel nets were weighed and measured <br />and returned to the river as close to point of capture as possible. Any razorback sucker or <br />Colorado pikeminnow greater than 175 mm TL were implanted with a PIT tag unless previously <br />tagged. <br />Larval fishes of both species were sampled with quatrefoil light traps and/or seines. <br />Light traps were versions commercially produced by Southern Concepts (Birmingham, AL) <br />having slit widths of either 2, 4, or 6 mm with 4 mm being most common. Traps were set by <br />tying them to emergent aquatic or overhanging terrestrial vegetation at dusk and were retrieved <br />the following morning before dawn. Seine hauls were completed using a one person larval seine <br />(1 mZ, 0.8 mm mesh) or a beach seine (4 m X 1.5 m, 1.25 mm mesh). Seining was conducted in <br />late afternoon or early evening in association with setting of light traps. Larger fish captured in <br />seines were processed (identified and counted) in the field while smaller fish and light trap <br />samples were preserved in 100% ethanol and transported to the Larval Fish Laboratory, Colorado <br />State University, Ft. Collins, Colorado for processing. <br />Larval razorback sucker sampling in 1994 began in early May (coinciding with initial <br />captures of sucker larvae below the middle Green River spawning site} and ended in mid-June. <br />Sampling in 1995 extended from 19 April (when main channel temperature reached 14°C) until <br />mid-July. Sampling for Colorado pikeminnow larvae was conducted in late June. Due to <br />overlap in suspected spawning seasons, sampling for Colorado pikeminnow larvae coincided <br />with the latter part of razorback sucker larval sampling. Habitats sampled with light traps <br />included zero velocity nursery areas including main channel backwaters and flooded mouths of <br />tributaries and ephemeral washes. Seine hauls were also conducted in low to zero velocity <br />habitats. <br />3 <br />