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i • ~ ... <br />Methods: Obtain a series of ontogenic stages for both species and <br />determine structures microscopically. <br />Task: Determine neuroanatomy of sensory system: when do connections become <br />functional (i.e. "turned on"). <br />Methods: Obtain a series of ontogenic stages for both species and <br />determine structures microscopically. <br />H1: Breeding tubercles are important reproductive organs that may be involved <br />in attracting mates and influence the successful completion of spawning. <br />Task: Evaluate the role of tubercles in reproduction. <br />Methods: Obtain tissues from sexually-mature fish and examine these <br />structures microscopically to determine possible function. <br />Population Augmentation or Re-establishment <br />Knowledge of genetic control and olfactory response to imprinted odors can <br />assist in recovery of Colorado squawfish and razorback sucker by augmenting <br />existing populations, or establishing new populations of the fishes. This will <br />require incorporation of new ideas into fisheries management, and long-term <br />field efforts to successfully augment or reestablish new populations. <br />We propose that the larger, and presumably more stable populations in the <br />Yampa-Green system be evaluated and studied for population attributes, and to <br />determine relationships between fish response and environmental conditions. <br />Although some "natural" imprinting would be evaluated in Colorado squawfish <br />and razorback sucker in the Green River, other locations would be more <br />acceptable for augmentation and re-establishment. Population augmentation <br />should be attempted in the Colorado River, by utilizing existing knowledge. <br />Reestablishment and testing of synthetic substances might be best conducted <br />in the San Juan River. Finally, an effort to reestablish the fishes in <br />locations where they currently do not exist, perhaps in the lower basin, would <br />ultimately test the effectiveness of the developed methods. Because of time <br />required to test the previously mentioned hypotheses, it would be best to <br />conduct all studies simultaneously, and to make adjustments in the program, as <br />needed, when data become available. <br />VII. Accomplishments to date: <br />Experts identified, study plans formulated- March 30. <br />Draft Study Plan submitted May 4, 1990. <br />Work activities suggested for FY 90. <br />Scope of Work for FY 1991 provided. PI's, study locations, etc. would <br />be identified by the Work Group about 60 days after project approval. <br />VIII. Recommendations for FY 1990 work: <br />9 <br />