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~ ~. <br />Prior collections indicated that field indentification of Gila spp. <br />was diddicult. Therefore, specimens collected at Coal Creek were <br />transported to Vernal for use in evaluating all known methods of species <br />identification. <br />Different morhpological counts and measurements presented by various <br />authors (Smith et al 1979; Minckley 1973) were made on live and pre- <br />served specimens. Taxonomists and knowledgeable field workers were also <br />contacted during the interpretation of the results of this work.' In <br />addition, selected specimens were sent to noted authorities for identification. <br />Although all specimens were not sacrificed, a cm grid board was utilized <br />for photographing all Gila spp. for future study. During one collection <br />trip, fin tissue was taken from all collected Gila spp. and shipped to <br />Arizona State University for electrophoretic analysis. <br />Sacrificed specimens were subjected to a taxonomic study. Speci- <br />mens were x-rayed and vertegral counts were made. Eight meristic <br />characters and gross morphological shape- were used to segregate species. <br />These characters were weighed according to their merits for species <br />segregation since considerable overlap occurs bwtween species for part- <br />iclar meristic characters (e.g. precaudal vertebrae counts for G. c pha <br />and G. robusta). Meristic characters exhibiting the least overlap were <br />weighed more than those with considerable overlap as follows: <br />Character Weighing Factors <br />1. Mouth, whether terminal or inferior 2 <br />2. Eye diameter 1 <br />3. Gill rakers counts on second arch 4 <br />4. Precaudal vertebrae 2 <br />5. Dorsal and anal fin ray number 1 <br />10 Totals <br />6. Head length divided by cuadal peduncle depth 3 <br />7. Caudal peduncle length divided by head length 2 <br />8. Total vertebrae count 5 <br />10 Totals <br />Characters 1 through 5 gave the best separation into the three <br />species. Characters 6 through 8 were utilized to separate G. cypha from <br />G. elegans if meristic characters 1 through 5 did not segregate the two. <br />Four Vernal FWS fishery biologists then participated in the identi- <br />f ication of specimens and a score sheet was compiled for each specimen. <br />A specimen that received 7.5 out of 10 points (75y) was required in <br />order to classify a specimen into one of the species; less than 7.5 was <br />considered an unknown. <br />