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<br />within each cell produced few additional fish. The usefulness of the lengthy but more complete depletion <br />~ effort was marginal compared to the untreated habitat area, suggesting modifications to the sampling <br />protocol were necessary. <br />In 1999 sampling intensity was reduced in individual backwaters by eliminating individual cells <br />~ and expanding removal efforts to cover the full 50-mile (80.5 km) reach. During the first three trips in <br />1999, at least two removal passes were made in each of these large flooded habitats. A series of seine <br />hauls was made in each habitat until all seinable area was covered at least twice; however, during the <br />R final two weeks, the seinable area was covered only once with a series of hauls due to time constraints. <br />In 1999 exclusion block nets were installed in three selected flooded wash habitats (School Bus Wash, <br />White Wash, and Red Wash). These habitats were selected because they were too large and deep to be <br />M effectively seined, but could be easily blocked. Block nets were 6.4 mm plastic mesh suspended from <br />15.9 mm steel rebar and 6.4 mm shock cord by zipties. The block nets were 2 to 3 m high and $ to 15 m <br />wide, depending on the width of the habitat. Nets were installed inside the mouth of the washes while <br />they were dry. When the washes became inundated at higher discharge, the block nets allowed larvae <br />and smaller fishes to pass through the netting but excluded larger fishes, creating an area that had <br />potentially fewer predators and less competition for resources. In 2000, the Program recommended <br />discontinuation of the block nets; thus, all habitats were sampled similarly as in 1999, but no habitats <br />were blocked. <br />2.2.2 Colorado River Methods <br />In the Colorado River, removal efforts were intended primarily to benefit Colorado pikeminnow <br />and other native species. Colorado pikeminnow are known to spawn on the descending limb of the <br />hydrograph in the Colorado River above Loma near RM 154 (Anderson 1999) and possibly just below the <br />Colorado/Utah state line, or below Westwater Canyon (Trammell and Chart 1999d). Once hatched, <br />larvae from these spawning sites drift downstream into the sampling reaches chosen for removal efforts. <br />Removal efforts were conducted after the spring peak flows for four weeks in 1998 and 1999, and for five <br />weeks in 2000. The sampling periods generally began well after the peak flow because no backwater <br />-6- <br />