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Last modified
7/14/2009 5:02:35 PM
Creation date
5/22/2009 7:28:56 PM
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UCREFRP
UCREFRP Catalog Number
9361
Author
Beyers, D. W. and C. Sodergren.
Title
Assessment and Prediction of Effects fo Selenium Exposure to larval Razorback Sucker.
USFW Year
1999.
USFW - Doc Type
Project 95/CAP-6 SE,
Copyright Material
NO
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of 12 cm and height of 15 cm. Depth of test solutions was 9.5 cm. Cool-white fluorescent lamps <br />were the only source of illumination (530 Ix), and a 12:12-h light:dark photoperiod was <br />maintained. <br />Larvae were fed an average ration of 886 control or selenium-enriched rotifers daily <br />based on their respective experimental treatment. Survival of fish in each treatment was <br />monitored daily. Growth was quantified at the end of the 28-d exposure period by determining <br />the average blotted wet mass and average total length (TL) of fish that survived. Average mass <br />was measured to 0.0001 g; TL to 0.1 mm. <br />To compare sensitivity of razorback sucker and fathead minnow to dissolved selenium, <br />96-hour renewal-acute toxicity tests were simultaneously conducted with each species using <br />methods prescribed by the American Society for Testing and Materials (1990b). Experimental <br />treatments were assigned to replicate beakers (n=4) using a randomized, balanced design with <br />seven target exposure concentrations (0.0, 6.38, 12.8, 25.5, 51.0, 102, and 204 mg/L selenium). <br />Fifteen larvae were randomized to each beaker (20 larvae per beaker were inadvertently used in <br />the razorback sucker test). Range-finding and 96-hour renewal-acute tests were conducted using <br />1-L polyethylene beakers containing 0.50 L of exposure solutions. Depth of test solutions was <br />7.3 cm. Test solutions were renewed every 24 h. Cool-white fluorescent lamps were the only <br />source of illumination (530 lx), and a 12:12-h light:dark photoperiod was maintained. <br />Larvae were offered live <_ 24-h-old brine shrimp nauplii before and during toxicity tests. <br />During a test, brine shrimp nauplii were introduced into exposure beakers three times daily and at <br />least 2 hours before renewal. Survival was monitored at 6, 12, 24, 48, 72, and 96 h after start of <br />the exposure period. <br />5 <br />
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