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h; temperatures were highest from 1300 to 1900 h (e.g., 24.5°C for the 22°C fluctuating <br />treatment); and cooling occurred at a constant rate from 1900 to 0100 h. Water <br />temperatures in the constant and diel fluctuating regimes were maintained close to the <br />desired nominal or nominal + 2.5°C diel range (Table 1). Head reservoirs provided <br />uniformly heated water to each incubation chamber. Flow-through conditions in each <br />incubation chamber (complete exchange rate every 7.6 min) maintained dissolved oxygen at <br />5-6 mg/L, and prevented accumulation of waste products. Photoperiod was 14 h light:l0 h <br />dark. Some embryos became infected with fungus in the first 16 h after tests began and <br />embryos in all test chambers were immediately treated with 1 % formalin for 5 min and, 2 h <br />later, with 5 mg/L malachite green for 30 min, A few embryos (< 5%), died early in the <br />study, perhaps from fungus or developmental deficiencies. They were replaced with viable <br />embryos from the appropriate fifth incubation chamber. After the first 16 h, no embryos <br />were replaced. Fungus was not noted after treatment with malachite green and all <br />subsequent egg mortality was attributed to treatment effect. Treatment of embryos with <br />fungicide was not seen as a confounding factor because all embryos were treated similarly. <br />Embryos with an opaque, irregularly-shaped chorion were pronounced dead; mortality was <br />verified under a dissecting microscope (30x). <br />Embryos were monitored 6-12 times daily and first, 50%, and 100% hatch were recorded. <br />Immediately after hatching, each larva was measured (total length, TL, to the nearest 0.1 <br />mm) with a dissecting microscope fitted with a calibrated ocular micrometer. Measured <br />larvae were counted and placed in 0.5 L jars in water baths at treatment temperature until <br />6 <br />