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and no hatch at the other temperatures. Optimum embryo incubation temperature for <br />Colorado squawfish was determined to be 20-22°C (Hamman 1981; Marsh 1985). <br />The narrow optimum temperature range for Colorado squawfish embryo incubation <br />suggested by laboratory studies was not expected given the highly variable temperature <br />regimes found during the spawning season of this species. Colorado squawfish in the <br />unregulated Yampa River, Colorado, may spawn when water temperatures range from 16 to <br />26°C (Nester et al. 1988, Tyus 1990). The relatively wide temperature range during <br />presumed reproduction by Colorado squawfish in the wild suggests that the optimal <br />temperature range for embryo incubation found in previous laboratory studies may be too <br />narrow. <br />This study was conducted to determine effects of water temperature and of constant and <br />fluctuating temperatures on hatch of embryos, and on survival and development of <br />Colorado squawfish larvae. <br />Methods <br />Colorado squawfish embryos were obtained from the Dexter National Fish Hatchery and <br />Technology Center, Dexter, New Mexico. Embryos used in the 18-26°C treatments in 1991 <br />were from wild fish captured from the Colorado River, near Grand Junction, Colorado. <br />The wild adults were held in ponds and allowed to ripen naturally until 14 June, which is <br />0-4 weeks earlier than wild Colorado squawfish spawn in upper Colorado River basin <br />(Nester et al. 1988; Tyus and Haines 1991). Embryos used in the 30°C treatment in 1992 <br />were from a mixed, upper Colorado River broodstock held in the Dexter hatchery; these <br />4 <br />