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Last modified
7/14/2009 5:02:36 PM
Creation date
5/22/2009 7:26:34 PM
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UCREFRP
UCREFRP Catalog Number
9572
Author
Bestgen, K. R.
Title
Interacting Effects of Physical and Biological Factors on Recruitment of Age-0 Colorado Squawfish.
USFW Year
1997.
USFW - Doc Type
Fort Collins, CO.
Copyright Material
NO
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treatment); and cooling occurred at a constant rate from 1900 to 0100 h. Water <br />temperatures in the constant and diel fluctuating regimes were maintained close to the <br />desired nominal or nominal + 2.5°C diel range (Table 1). Head reservoirs provided <br />uniformly heated water to each incubation chamber. Flow-through conditions in each <br />incubation chamber (complete exchange rate every 7.6 min) maintained dissolved oxygen <br />at 5-6 mg/L, and prevented accumulation of waste products. Photoperiod was 14 h <br />light: l0 h dark. Some embryos became infected with fungus in the first 16 h after tests <br />began and embryos in all test chambers were immediately treated with 1 % formalin for 5 <br />min and, 2 h later, with 5 mg/L malachite green for 30 min. A few embryos (< 5%), died <br />early in the study, perhaps from fungus or developmental deficiencies. They were <br />replaced with viable embryos from the appropriate fifth incubation chamber. After the <br />first 16 h, no embryos were replaced. Fungus was not noted after treatment with <br />malachite green and all subsequent egg mortality was attributed to treatment effect. <br />Treatment of embryos with fungicide was not seen as a confounding factor because all <br />embryos were treated similarly. Embryos with an opaque, irregularly-shaped chorion <br />were pronounced dead; mortality was verified under a dissecting microscope (30x). <br />Embryos were monitored 6-12 times daily and first, 50%, and 100% hatch were <br />recorded. Immediately after hatching, each larva was measured (total length, TL, to the <br />nearest 0.1 mm) with a dissecting microscope fitted with a calibrated ocular micrometer. <br />Measured larvae were counted and placed in 0.5 L jars in water baths at treatment <br />temperature until hatching was completed. Surviving larvae were then counted and <br />returned to their incubation chambers. <br />12 <br />
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