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SPAWNING OF HUMPBACK AND ROUNDTAIL CHUBS <br /> 3 <br />J= <br /> 2 <br /> 1 <br /> U 0 <br /> <br /> -1 <br /> /i <br /> ~I <br /> 0` <br /> n <br />- L i"' <br /> u <br /> U <br /> a <br /> -3 <br />• BONYTAIL <br />^ HUMPBACK <br />° ° UNCLASSIFIED <br />° ROUNDTAIL <br />^ ^ <br />^^ ^ <br />o ^ ^ ^ <br />O ^ ~ p ^ ^ <br />^ ° ^ ^ ° <br />o ^ ^ <br />^ 8 m e ^ ° <br />o &° ^ ~ ~ ° ° °° ^ ^ <br />° ,~ ^ ^ ° ~ ~ <br />o^ ~ °^ e ^ <br />e ^0 6 ~g 8 <br />^ o ^ ^ o^ p e o ° ^ <br />e 9c o <br />o eo ^q]^ o <br />o e^o e ° ° ~ ^ °O <br />e ~ ° e p m ° <br />g 9 c o ° ~^ e <br />o e°° <br />o e o pee o n e e e e e e <br />e <br />e o 8 m° e ° ~n <br />e e oe eed' so Ae°o e o 0 <br />n ° ° cP9° o e a e e e e <br />° ° e ° o ~H a ~w~ e °~ e+ <br />0 <br />o rP ° e~ e <br />° Se ee ee c° ,0 <br />m ~ ° <br />e ° o e ° o ne ~ 4Q ~e a °9e e e ee <br />° ° e e ° e6, me e o <br />o ~. e° o e o eee ,p e ° ° <br />~ e <br />o d° eo 0 <br />° o °o ° ° o <br />00 <br />e <br />• <br />PC I = bb°~ <br />137 <br />-3 -2 -1 0 1 2 3 <br />PC I <br />FlcuxE 1.-Principal component (PC) scores for 597 fish captured in the Colorado River at Black Rocks, <br />Colorado, 1983-1985, and classified in the field as either humpback chub, roundtail chub, bonytail, or unclassified <br />Gila sp. Within the limits of the polygon there were 146 (54%) humpback chub scores that are not individually <br />plotted for purposes of graphical clarity. The line Y = -0.123 X, fitted by eye, is shown; Y is PC II, and X is PC <br />I. <br />Results <br />Analyses of Morphologic and Meristic Characters <br />During the 3-year study, we identified 597 ma- <br />ture Gila spp. in the field. Of these, we considered <br />270 as humpback chub, 269 as roundtail chub, 1 <br />as a bonytail (Kaeding et al. 1986), and 57 as un- <br />classified Gila sp. The principal component anal- <br />ysis of the morphologic measurements revealed <br />two clusters, humpback and roundtail chubs (Fig- <br />ure 1). The first two principal components (PC I <br />and II) explained 85% of the variation in the data <br />matrix. The line Y = -0.123 X, fitted by eye, <br />divided the two clusters. Because the slope of this <br />line is near zero, the in$uence offish size on shape <br />was almost entirely accounted for in PC I, and the <br />shearing technique of Humphries et al. (1981) was <br />not needed (G. R. Smith, University ofMichigan- <br />Ann Arbor, personal communication). In analyses <br />of Colorado River Gila spp. that included shorter <br />specimens, such lines had positive slopes near un- <br />ity (e.g., Bookstein et al. 1985). The gradual slope <br />of our line may result from the relatively narrow <br />length-class of adult fish examined. <br />The most important morphologic characters for <br />distinguishing species were depth of the nuchal <br />depression and caudal peduncle depth (Table 1). <br />Of the taxonomic assignments made in the field, <br />