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1608 JOURNAL FISHERIES RESEARCH BOARD OF CANADA, VOL. 29, NO. 11, 1972 <br />In addition to voltage, pulse width affects the responses <br />of fish (Wary ] 9S6). To determine the range of pulse <br />widths most desirable for the experiments, a series of <br />tests were conducted on spot to determine the times to <br />swim one lap at 1.5 vJ10 cm with 25 and 30 pulses/sec <br />at pulse widths of 0.7, 1.2, and 2.0 msec (Table 2). <br />At a pulse rate of 25/sec, times ranged from 3.3 to 4.6 <br />sec. At 35 pulses/sec, however, it took an average of <br />5.4 sec to swim one lap at a pulse width of 0.7 msec, <br />and 3.2 sec at a 1.2 msec pulse width. These differences <br />were tested by analysis of variance, which clearly showed <br />no difference in response times at 35 pulses/sec (F = <br />2.2G < F.95 (~.z7) = 3.36). Because of the closeness <br />in response times at 25 pulses, no analysis of variance <br />was needed. <br />TABLE 2. Times (sec) to swim one lap (4.5 m) <br />at three pulse widths for spot stimulated with <br />1.5 v/10 cm at two pulse rates. <br />Pulse rate <br />Pulse width <br />(msec) 25 35 <br />The responses of fish subject to pulse widths ranging <br />from 0.7 to 2.0 msec were similar; hence, the test ani- <br />mals were subjected to pulse widths within this range. <br />Effective Combinations <br />The combinations of electrical parameters which <br />are considered most desirable for inducing elec- <br />trotaxis and for leading 12 of the 13 species are sum- <br />marized in 'Table 3. Little difference existed between <br />the electrical parameters for coastal pelagic and <br />bottom fish, except for croakers, as control was not <br />obtained for this species. However, response times <br />to swim one and two laps and average distance <br />covered differed greatly between the two groups as <br />bottom fish swam at much slower speeds and could <br />not be led as far as the coastal pelagics. <br />The optimal electrical parameters were similar for <br />Longspine porgy and spot; the only difference was <br />that spot could be effectively controlled at a slightly <br />wider range in repetition rate. Croaker could not be <br />eIL'cc[ively controlled (Table 4). <br />Longspine porgy reaction time curves to swim one <br />lap were similar in shape but not in amplitude for <br />1.5, 3.0, and 4.5 v/10 cm (Fig. 2). The similarity <br />in shape indicates that voltage has little effect. A <br />decrease in pulse rate increased the time to swim <br />to the anode. Combinations of high voltage and <br />high pulse rates were undesirable for inducing <br />electrotaxis in both Longspine porgy and spot. <br />0.7 4.6~i.lia•b 5.42.09 <br />1.2 4.6f1.22 3.2f0.88 <br />2.0 3.30.41 4.4f1.74° <br />BAverage and 95°Jo confidence interval. <br />bThirty fish tested; 10 in other tests. <br />°Nine fish responded. <br />TAIILE 3. Effective combinations of voltage (per 10 cm) and pulse rate (per sec) for <br />inducing electrotaxis in the species studied and the approximate amperes (per m2). <br /> <br />Species <br />(v) <br />Pulses <br />(Amp) Fish length (mm) <br />Range Avg. <br />Coastal pelagics <br />Scaled sardine 1.5 IS-SS 62.5 100-145 118 <br /> 3.0 8-28 86.5 100-145 118 <br />Spanish sardine 1.5 35-45 43.3 85-180 130 <br /> 3.0 15 86.5 85-180 130 <br />Round herring 3.0 2515 86. S 80-150 104 <br />Silver anchovy 3.0 35-45 98.3 85-110 96 <br />Butterfish 1.5 35 41.3 80-100 118 <br /> 3.0 45 86.5 80-160 118 <br />Chub mackerel 1.5 15 53.8 125-240 180 <br />Bumper 1.5 15 57.0 135-230 173 <br />Rough scad 1. S 15-2S 42.3 120--145 133 <br />Thread herring 1.5 15 62.5 80-185 146 <br />Round scad 1.S 15 43.3 90-170 148 <br />Bottom frsh <br />Spot 1.5 15-35 43.3 90-250 120 <br />Longspine porgy 1.5 25-35 43.3 100-160 128 <br />