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levels in the blood doubled immediately after the fish were. shocked and remained high for <br />one hour and recovered to preshock levels after approximately six hours. The rapidity of <br />lactate increase after shocking reflects the severity of the period of anaerobic muscular <br />activity. Schreck's et a1. (1976) lactate increases after shocking were much the same as <br />those found by other researchers for different species (Johnson et al. 1956, Caillouet 1967, <br />Burns 1978). The recovery is also similar to that observed in rainbow trout that have <br />undergone strenuous muscular excerise (Black et al. 1960). Other blood chemistry changes <br />occur as a result of shocking. For example, glucose follows a reaction similar to lactate <br />except the increase is not as abrupt and recovery takes longer (up to 12 hours) (Schreck <br />et al. 1976). The U. S. Environmental Protection Agency has recommended that fish collected <br />by electrofishing not be used in physiological or bioassay studies because of blood <br />chemistry changes (Weber 1973). <br />2t <br />15 <br />g <br />E <br />m 1C <br />0 <br />V <br />5 <br />Time AFter Shock (h) <br />Fi:gure 5. Average concentrations (mg/100 ml) of lactate (vertical <br />line at top of each bar shows range) levels in plasma <br />of electroshocked and unshocked rainbow trout (adapted <br />from Schreck et al. 1976). <br />Fish differ greatly in their ability to carry oxygen in the blood (Table 3). The amount of <br />oxygen in the blood can play an important role in breaking down metabolites released into <br />the blood following electrofishing or in determining the overall vitality of a fish. <br />Temperature of the water (the cooler the better) and life stage of the fish can also affect <br />the amount of oxygen in the blood. <br />CAL-NEVA WILDLIFE TRANSACTIONS 1984 <br />65 <br />0 1 3 ",j 6 <br />