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TABLE OF CONTENTS <br />(cont.) <br />Paste <br />Antibiotics ...................................... 5-19 <br />Equilibration Time ............................... 5-19 <br />Evaluation of Sperm Motility .......................... 5-20 <br />Restripping of Spawned Females ...........••••••••••••• 5-22 <br />Literature Cited (Spawning Techniques) ................ 5-23 <br />SECTION 6. TECHNIQIIES TO ENHANCE SPAWNING POTENTIAL ....... 6-1 <br />Hormonal Manipulation (induced maturation) ............ 6-1 <br />Luteinizing Hormone-Releasing Hormone analogs .... 6-2 <br />LHRHa "Fact Sheet" ............................... 6-3 <br />Literature Cited (Hormonal Manipulation) ......... 6-6 <br />Photoperiod Manipulation (induced maturation) ...•.•••• 6-8 <br />Literature Cited (Photoperiod Manipulation) ...... 6-11 <br />Semen Extenders ....................................... 6-13 <br />Literature Cited (Semen Extenders) ............... 6-15 <br />Cryopreservation of Semen ............................. 6-16 <br />Literature Cited (Cryopreservation of Semen) ..... 6-20 <br />SECTION 7. FERTILIZATION .................................. 7-1 <br />Wet Method ............................................ 7-1 <br />Dry Fertilization ..................................... 7-2 <br />Fertilization Medium (saline fertilization) .........•• 7-3 <br />Effect of Ovarian Fluid ............................... 7-5 <br />ix <br />