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F. Number of Spawning Takes <br />Time of spawning has been shown to be highly <br />heritable. ,Fish that. spawn at or near the same time <br />stand a good chance of being related. To avoid <br />possible inbreeding and loss of heterozygosity, <br />future brood should be represented by eggs obtained <br />from at least 60~ of the individual spawning takes. <br />Egg takes that include the beginning, middle, and <br />end of the spawning season. should all be <br />represented. As stated above, actual number of eggs <br />retained from each take should be relative to the <br />number of fish spawned on that occasion. <br />G. Crossbreeding (outcrosses) <br />As stated previously, a l0~ infusion of genetic <br />material from the founding population every 10 years <br />will help to minimize domestication. Outcrosses <br />with related "strains" may also be considered as a <br />method to preserve or increase variability. <br />However, before such measures are .initiated, <br />documentation of need should be supported by <br />electrophoretic data, as well as consultation with <br />trained geneticists. <br />H. Selection <br />The best methodology to minimize selection is to <br />not select at all during spawning operations. With <br />the possible exception of fish that suffer obvious <br />deformities, all fish .should be spawned. Fish <br />should never be selected based on size, coloration, <br />body condition, etc. <br />I. Mating Regimen <br />Individuals to be mated should always be selected at <br />random. To avoid selection for early maturity, mate <br />individuals only at the time when 90~ of the <br />population becomes-sexually mature. If there is any <br />doubt about the quality of a particular male; use <br />another male to fertilize the eggs of a given <br />female. Only fish with obvious deformities should <br />be excluded from contributing to future. generations. <br />1-7 <br />