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2. Plan Management Practices <br />All practices that involve intentional selection should be <br />avoided. It must be remembered that traits that relate to <br />performance in the wild cannot be selected for in the hatchery <br />environment. Unintentional selection may at times be <br />unavoidable, but it should be eliminated to whatever extent <br />possible. New, innovative practices may be required <br />to maintain as much genetic variation as possible in "wild" <br />broodfish populations. Dependent upon results of genetic <br />evaluation, there may be a need to go back to the original <br />founder population for an infusion of genetic material. <br />Allendorf and Leary, 1988 (2), have suggested that a 10~ <br />infusion of genetic material from the founding population <br />every 10 years may be sufficient to effectively minimize <br />domestication. There is no substitute for long range planning <br />in an effort to conserve genetic variability. All management <br />practices should be evaluated based on their genetic impact <br />prior to adoption as part of hatchery procedure. <br />3. Methods to Minimize Loss of Variability `in Future Brood <br />Populations <br />A. Mating Ratio <br />The optimal procedure to prevent loss of variability <br />is to use paired coatings between one female and one <br />male. Paired coatings function to increase the <br />effective population size. If paired coatings are <br />not possible, every attempt should be made to <br />include a genetic contribution from all individuals <br />in the population. <br />B. Avoid Inbreeding <br />The closer the relationship between the parents, the <br />less variability in the genetic make-up of the <br />offspring. One method to reduce the degree of <br />inbreeding is to use coatings between males and <br />females of different year classes. This methodology <br />will reduce loss of variability as well as prevent <br />the reproductive isolation of year classes. <br />C. Number of Matings <br />The recommended number of paired coatings to maintain <br />a broodstock is 250 (Leary, personal commumication). <br />The minimum number of paired coatings to maintain an <br />acceptable level of variability is 100 (3). These <br />numbers refer to the number of fish pairs required <br />to contribute gametes to the next generation. In <br />1-4 <br />