7188

• UCREFRP Catalog Number: 7188
• Author: Muth, R. T., C. M. Haynes and C. A. Carlson
• Title: Culture of Roundtail Chub,
• USFW Year: 1985
• Copyright Material: YES

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AGE(DAYSI FROM HATCHING
Ftc. 1.-Summary of growth and development through the larval period Eor roundtail chub
reared at 19-20°C in flow-through troughs at Colorado State University, Fort Collins, 1983. Verti-
cal line is the size range and aoss line is the mean. Horizontal line represents range of ages and
sizes over which each developmental event and phase was observed.
with a mean of 2.8 mm (n=50). After water hardening, eggs were transported to Facilities at Colo-
rado State University and placed on wire-screen trays in aflow-through incubator (Heath Tecna
Corp., Kent, Washington). Water en[ered the incubator at 12.3 1/min. During incubation, dis-
solved oxygen ranged from 7.5 to 8.0 mg/1 (100 to 105% sat.), pH Erom 7.3 to 7.7, total water
hardness from 410 to 440 mg CaCOJ/1 and water temperature was 19°C. Samples of embryos were
collected every 4 h, fixed in 10% buffered (ormalin and preserved in 3~ buffered (ormalin. Notable
embryological events (stages conforming to Balinsky's [Prot. Zool. Soc. London, 118:335-344, 1948]
convention) at specific ages were:
4 h-beginning of cleavage (Stage 3),
20 h-blastula (Stage ] 1).
36 h-late gastrula (Stage 14),
44 h-late neurula (Stage 16),
56 h-oval eyes (Stage 19),
60 h-early tail-bud (Stage 20),
92 h-fin[old (Stage 23 ),
108 h-pigmented eyes (Stage 24).
Hatching began at 126 h and was completed by 156 h; approximately 94ro of the eggs hatched.
Newly hatched larvae were immediately transferred to two flow-through troughs (3.0 x 0.3 x 0.2
m), which received water at a rate of 6.0 I/min. Throughout the rearing period, dissolved oxygen
ranged from 8.0 to 9.0 mg/1 (105 to 115 sat.), pH from 7.1 to 7.9, total water hardness from 450
to 470 mg CaCOJ/1 and water temperature from 19 to 20° C. After swim-up, larvae were fed three
to [our limes daily with brine shrimp nauplii and commercial TetraMin Fry Diet and S[aple Food
(Tetra Corp., West Germany). Fish were sampled every 24 h (n=50/sample) during the first 19
days of rearing. Thereafter, samples were taken every 120 h (n=50/sample). Larvae were fixed in
)0% buffered (ormalin preserved in 3% buffered [ormalin. All specimens were measured (total
Notes
M'cTALARVAE
153
JUVENILE `-'
V