668
<br />NOTES
<br />Tetracycline marks may persist for at least sev-
<br />eral years in bones and otoliths (Weber and Ridg-
<br />way 1967; Blacker 1974). Muth et al. (1988) ob-
<br />served no loss in quality of TC marks in otoliths
<br />of Colorado squawfish larvae held indoors up to
<br />77 d after marking. Tsukamoto (1985) found that
<br />larvae of ayu Plecoglossus altivelis reared in a
<br />greenhouse retained TC marks in their otoliths for
<br />at least 164 d after marking. Tetracycline marks
<br />were also retained in otoliths of ayu recaptured 2
<br />months after larvae had been released in the sea
<br />(Tsukamoto 1988). Results of tests by Lorson and
<br />Mudrak (1987) to determine effects of sunlight on
<br />tetracycline marks in otoliths of young American
<br />shad showed that overall mark retention 39 d after
<br />marking was similar between fish held in indoor
<br />or outdoor production tanks. However, contrary
<br />to our short-term findings, they noted that marks
<br />in otoliths of fish held outdoors were somewhat
<br />less clear and intense. A reduction in percent of
<br />fish marked (presumably due to loss of mark) be-
<br />tween days 39 and 134 after marking was also
<br />observed among fish held outdoors; no compa-
<br />rable data for fish held indoors were provided.
<br />Experiments may be necessary to assess the long-
<br />term durability of otolith marks in Colorado
<br />squawfish.
<br />Acknowledgments
<br />This paper is contribution 51 of the Larval Fish
<br />Laboratory, Colorado State University. Funding
<br />was provided by the U.S. Bureau of Reclamation,
<br />Upper Colorado Regional Office, under coopera-
<br />tive agreement 8-FC-40-06460. We thank D. Be-
<br />yers, C. Carlson, A. Powell, D. Snyder, and an
<br />anonymous reviewer for reviewing the manu-
<br />script.
<br />References
<br />Blacker, R. W. 1974. Recent advances in otolith stud-
<br />ies. Pages 67-90 in F. R. Harden Jones, editor. Sea
<br />fisheries research. Wiley, New York.
<br />Brothers, E. B. 1985. Otolith marking techniques for
<br />the early life history stages of lake trout. Great Lakes
<br />Fishery Commission, Research Completion Re-
<br />port, Ann Arbor, Michigan.
<br />Haines, G. B., and H. M. Tyus. 1990. Fish associations
<br />and environmental variables in age-0 Colorado
<br />squawfish habitats, Green River, Utah. Journal of
<br />Freshwater Ecology 5:427-~35.
<br />Hamman, R. L. 1981. Spawning and culture of Col-
<br />orado squawfish in raceways. Progressive
<br />Fish-Culturist 43:173-177.
<br />Hettler, W. F. 1984. Marking otoliths by immersion
<br />of marine fish larvae in tetracycline. Transactions
<br />of the American Fisheries Society 113:370-373.
<br />Kaeding, L. R., and D. B. Osmundson. 1988. Inter-
<br />action of slow growth and increased early-life mor-
<br />tality: an hypothesis on the decline of Colorado
<br />squawfish in the upstream regions of its historic
<br />range. Environmental Biology of Fishes 22:287-298.
<br />Koller, L. R. 1965. Ultraviolet radiation. Wiley, New
<br />York.
<br />Lehninger, A. L. (975. Biochemistry. Worth, New
<br />York.
<br />Lorson, R. D., and V. A. Mudrak. 1987. Use of tet-
<br />racycline to mark otoliths of American shad fry.
<br />North American Journal of Fisheries Management
<br />7:453-455.
<br />Muth, R. T., and T. P. Nesler. 1989. Marking Colo-
<br />rado squawfish embryos and newly hatched larvae
<br />with tetracycline. Southwestern Naturalist 34:432-
<br />436.
<br />Muth, R. T., T. P. Nesler, and A. F. Wasowicz. 1988.
<br />Marking cyprinid larvae with tetracycline. Ameri-
<br />can Fisheries Society Symposium 5:89-95.
<br />Nesler, T. P., R. T. Muth, and A. F. Wasowicz. 1988.
<br />Evidence for baseline flow spikes as spawning cues
<br />for Colorado squawfish in the Yampa River, Col-
<br />orado. American Fisheries Society Symposium 5:
<br />68-79.
<br />Tsukamoto, K. 1985. Mass-marking of ayu eggs and
<br />larvae by tetracycline-tagging of otoliths. Bulletin of
<br />the Japanese Society of Scientific Fisheries 51:903-
<br />911.
<br />Tsukamoto, K. 1988. Otolith tagging of ayu embryo
<br />with fluorescent substances. Bulletin of the Japanese
<br />Society of Scientific Fisheries 54:1289-1295.
<br />Tsukamoto, K., and T. Kajihara. 1987. Age determi-
<br />nation of ayu with otolith. Bulletin of the Japanese
<br />Society of Scientific Fisheries 53:1985-1997.
<br />Tyus, H. M., and C. A. Karp. 1989. Habitat use and
<br />stream flow needs of rare and endangered fishes,
<br />Yampa River, Colorado. U.S. Fish and Wildlife
<br />Service Biological Report 89(14).
<br />Weber, D. D., and G. J- Ridgway. 1967. Marking Pa-
<br />cific salmon with tetracycline antibiotics. Journal of
<br />the Fisheries Research Board of Canada 24:849-
<br />865.
<br />Wydoski, R., and L. Emery. 1983. Tagging and mark-
<br />ing. Pages 215-237 in L. A. Nielsen and D. L. John-
<br />son, editors. Fisheries techniques. American Fish-
<br />eries Society, Bethesda, Maryland.
<br />Received October 9, 1990
<br />Accepted February 21, 1991
<br />
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