7827

• UCREFRP Catalog Number: 7827
• Author: Minckley, W. L., D. G. Buth and R. L. Mayden
• Title: Origin of Brood Stock Allozyme Variation in Hatchery-Reared Bonytail, an Endangered North American Cyprinid Fish
• USFW Year: 1989
• USFW - Doc Type: Transactions of the American Fisheries Society
• Copyright Material: YES

ALLOZYME VARIATION IN BONYTAIL
133
TwstE. 1.-Enzymes and nonspecifically stained proteins, loci, tissue sources, and electrophoretic conditions for
genetic evaluation of bonytails.

Enzyme or other protein

Code numbers

Locus

Tissue source Electrapho-
retic condi-
tionb
Acid phasphatase 3.1.3.2 Acp-1 Liver, brain A
Aconitate hydratase (mitochondriaq 4.2.1.3 mAcoh-A Muscle A
Aconitate hydratase (cytosolic) 4.2.1.3 sAcoh-A Liver A
Adenosine deaminase 3.5.4.4 Ada-1 Muscle B
Adenylate kinase 2.7.4.3 Ak-A Muscle C
Alcohol dehydrogenase 1.1.1.1 Adh-1 Liver D
Aspartate aminotransferase (mitochondria!) 2.6.1.1 mAat-A Muscle E
Aspartate aminotransferase (cytosolic) 2.6.1.1 sAat-A Muscte E
Calcium-binding protein Nonspecific Cbp-1~ Muscle E
Calcium-binding protein Nonspecific Cbp-2° Muscle E
Catalase 1.1 1. L6 Cat-1 Liver D
Creative kinase 2.73.2 Ck-A Muscle E
Cytosol aminopeptidase 3.4.1 I.l sAp-l Muscle E
Dipeptidase 3.4,13.! l Pep-Ad Muscle A, F
Dipeptidase 3.4.13.11 Pep-Fd Muscle A
Dipeptidase 3.4.13.11 Pep-Sd Muscle A
Esterase 3.1. L- Est-1 Muscle F
Esterase 3.1.1 - Est-2 Brain F
Fumarate hydratase 4.2.1.2 Fumh-1 Muscle A
General protein Nonspecific Gp-l~ Muscle E
Glucose-6-phosphate isomerase 5,3,1.9 Gpi-A Brain C
Glucose-6-phosphate isomerase 5.3.1.9 Gpi-B Muscle C
Glyceraldehyde-3-phosphate dehydrogenase 1.2.1.12 Gapdh-1 Muscle A
Glycerol-3-phosphate dehydrogenase 1.L1.8 Gapdh-A Muscle E
~-lditol dehydrogenase l.l.t.14 lddh-A Liver D
Isocitrate dehydrognease (mitochondriaq 1. L 1.42 mldh-A Muscle A
Isocitrate dehydrognease (cytosolic) I.I.1.42 sldh-A Muscle A
~-Lactate dehydrogenase I. L 1.27 Ldh-A Muscle A
~-Lactate dehydrogenase 1.1.1.27 Ldh-B Brain A
~-Lactate dehydrogenase 1.1.1.27 Ldh-C Liver A
Malate dehydrogenase (mitochondria!; NAD-dependent) 1.1.1.37 mMdh-A Muscle A, E
Malate dehydrogenase (cytosolic; NAD-dependent) 1.1.1.37 sMdh-A Muscle A
Malate dehydrogenase (cytosolic; NAD-dependent) 1.!.1.37 sMdh-B Muscle A
Malate dehydrogenase (mitochondria!; NADP-depen- L 1.1.40 mMdhp-A° Muscle A
dent)
Malate dehydrogenase (cytosolic; NADP-dependenq
1.1.1.40
sMdhp-A°
Muscle
A
Mannose-6-phosphate isomerase 53.1.8 Mpi-1 Muscle C
a-Mannosidase 3.2.1.24 aMan-1 Muscle C
Peptidase-C 3.4-- Pep-Cd Brain A
Phosphoglucomutase 5.4.2.2 Pgm-A Muscle C
Phosphogluconate dehydrogenase 1.1.1.44 Pgdh-1 Liver A
Praline dipeptidase 3.4.13.9 Pep-IJd Muscle A. F
Pyruvate kinase 2.7.1.40 Pk-1 Muscle E
Superoxide dismutase (cytosolic) 1.15.1. I sSnd-1 Muscle, liver C, A
Tripeptide aminopeptidase 3.4.1 1.4 Pep-Bd Muscle A
Xanthine dehydrogenase 1.1.1.204 Xdh-A Liver D
a IUBNC (1984).
n A = tris-citrate (Whitt 1970); B =phosphate-citrate (Selander et aL 1971); C = "Poulik" (Selander et al. 1971); D =borate
(Sackler 1966); E = histidine--citrate (Brewer 1970); F =lithium hydroxide (Selander of al. 1971).
~ Buth (1982).
a Frick (1983).
"Malic" enzyme.
known Muses, and six large fish captured in 1988
are now in good condition at Dexter. Because
bonytails from the lower Colorado basin cannot
be aged reliably by scale methods, identification
of these last individuals as either wild or hatchery-
produced is not possible unless they are killed.
However, otoliths of four of the five 1988 speci-
mens that died had six apparent annuli; therefore,
these fish most likely represent F, fish stocked in
1981. The other 1988 specimen was estimated to
be at least ! 0 years old. One of the three fish from
1986 was older than 20 years; otoliths of the other
two specimens were not examined. Based on
growth rates in hatchery ponds and in other arti-