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1096 <br />MODDE ET AL. <br />With the closure of Flaming Gorge Dam on the <br />Green River in 1963, the magnitude and duration <br />of floodplain inundation has been dramatically re- <br />duced (Flo Engineering 1996). However, because <br />little information exists on the early life history <br />needs of razorback suckers, few links between <br />those needs and floodplain habitat have been es- <br />tablished. The few juveniles captured in the upper <br />Colorado River basin were in the main channel <br />(Taba et al. 1965; Gutermuth et al. 1994). <br />The purpose of this study was to examine the <br />physical and biological characteristics of a manip- <br />ulated floodplain wetland in the middle Green Riv- <br />er and to describe the features that provide suitable <br />nursery habitat for juvenile razorback suckers. The <br />approach taken was to describe the environmental <br />conditions and fish use and to compare larval ac- <br />cess to floodplains with and without the effect of <br />Flaming Gorge Dam. The specific objectives of <br />the study were (1) to measure the environmental <br />variables that limit or influence the survival and <br />growth of age-0 fishes (i.e., dissolved oxygen, tem- <br />perature, depth, and zooplankton densities) in the <br />study wetland through the summer months, (2) to <br />determine the access of larval razorback suckers <br />to floodplains in general by comparing the timing <br />of larval drift with that of average bank-full flows, <br />and (3) to census the fishes occupying the study <br />wetland to determine whether razorback suckers <br />used the site. <br />Study Area <br />Larval razorback sucker collections were made <br />in the middle Green River between river kilome- <br />ters (rkm) 482 and 395 (measuring from the con- <br />fluence with the Colorado River; Figure 1). The <br />wetland sampled intensively was Old Charley <br />Wash (also known as Wood's Bottom), a 147-ha <br />floodplain depression on the Ouray National Wild- <br />life Refuge near Ouray, Utah, where a natural wet- <br />land was artificially partitioned into two units. His- <br />torically, water has entered the wetland via gravity <br />flow through an inlet canal (rkm 396) from the <br />Green River when discharge reaches 240 m3/s and <br />is connected to the river by flood flows over the <br />natural levee at approximately 396 ml/s. The inlet <br />canal was closed during this study, so that all water <br />entering Old Charley Wash was via overbank <br />flooding. Only the 80-ha main unit was studied. <br />The primary vegetation in the wetland was smart- <br />weed Polygonum sp. and sago pondweed Pota- <br />mogeton pectinatus, with small stands of bulrushes <br />Scirpus sp. and cattails Typha sp. around the mar- <br />gins. The study area was drained prior to winter <br />and filled each spring by flood flows. An outlet <br />structure at the downstream side was closed in <br />spring to retain water in the wetland and was <br />opened only to drain the wetland. The outlet struc- <br />ture included a concrete floor and walls lined with <br />polyethylene tubing filled with gravel, allowing <br />the efficient capture of fishes. <br />Methods <br />Environmental variables.-Maximum depth, <br />subsurface water temperature, dissolved oxygen, <br />and zooplankton densities were measured in Old <br />Charley Wash in spring and summer 1995 and <br />1996. Maximum depth was determined by sub- <br />tracting the elevation difference between the bot- <br />tom of the wetland and the reading on a staff gauge <br />on the outlet drain structure. Dissolved oxygen was <br />measured just before sunrise with a Hach DREL/ <br />2000 Water Quality Kit in 1995 and an OxyGuard <br />MkII meter in 1996 at a single location near the <br />center of Old Charley Wash. Zooplankton were <br />sampled from sites randomly selected from grids <br />superimposed over a map of the wetland. Subsur- <br />face temperature was taken from the first zoo- <br />plankton sample site on each date. Data were col- <br />lected approximately weekly while the river and <br />wetland were connected and biweekly thereafter <br />until the wetland was drained. Sampling dates <br />were between 31 May and 14 September in 1995 <br />and between 14 May and 8 July in 1996. Instan- <br />taneous river temperatures were recorded at the <br />U.S. Geological Survey gauge in Jensen, Utah <br />(rkm 500). <br />Zooplankton were collected in daylight with a <br />plankton net 10 cm in diameter with 243-µm mesh <br />by pulling the net just under the surface across a <br />known distance (8 m). Volume was determined by <br />multiplying the net diameter (10 cm) by the length <br />of haul. Samples were taken in three different di- <br />rections at each of three sites and preserved in a <br />5% solution of formalin. The total number of mi- <br />crocrustaceans per sample was estimated as fol- <br />lows: random grids were selected from a petri dish <br />14 cm in diameter with centimeter grids on the <br />bottom; up to 10% of the available grids were <br />counted, or a minimum of 200 zooplankters; and <br />the total number was calculated by direct propor- <br />tion (i.e., the number of zooplankters counted mul- <br />tiplied by the total area of grids divided by the <br />area counted). Relative density was estimated by <br />dividing the total number of zooplankters collected <br />by the water volume sampled. <br />Larval fish collections.-Razorback sucker lar- <br />vae were sampled in the middle Green River in <br />