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552 TRANS. AMER. FISH. SOC., 1991, NO. 3 <br />ACKNOWLEDGMENTS <br />We thank Roger E. Burrows, Adrien Ber. <br />nier, Fred W. Bitle, and Ross C. Kientz, of the <br />Bureau of Sport Fisheries and Wildlife, for <br />their suggestions and cooperation during this <br />research. <br />LITERATURE CITED <br />ANDREW F. J, P. C. JOHNSON, AND L. R. KERSEY. <br />1956. Further experiments with an electric screen <br />for downstream-migrant salmon at Baker Dam. <br />InL Pac. Salmon Fish. Comm., Progr. Rep. [11, <br />29 pp. (Processed.) <br />, L R. KERSEY, AND P. C. JOHNSON. 1955. An <br />investigation of the problem of guiding down- <br />stream-migrant salmon at dams. Int. Pac. Salmon <br />Fish. Comm., Bull. 8, 65 pp. <br />APPLEGATE, VERNON C., PAUL T. MACY, AND VIRGIL E. <br />HARRIS. 1954. Selected bibliography on the ap- <br />plications of electricity in fishery science. U. S. <br />Fish Wild]. Serv., Spec. Sci. Rept. Fish. 127, <br />55 pp- <br />IlmwET, A. M. R. 1959. Electric fishing with' pul- <br />satory direct current. New Zealand J. Sci. 2(1) <br />46-56. <br />BuBBows, ROGER E. 1951. A method for enumera- <br />tion of salmon and trout eggs by displacement. <br />Progr. Fish-Cult 13(1): 25-30. <br />COLLINS, G. B., C. D. VOLZ, AND R. H. LANDER. 1953. <br />The effectiveness of pulsating direct current in <br />controlling the movement of salmon fingerlings. <br />Bur. Commer. Fish., Biol. Lab., Seattle, Wash. <br />83 pp. (.Unpublished manuscript) <br />AND PARKER S. TREFETHEN. 1954. <br />Mortality of salmon fingerlings exposed to pulsat- <br />ing direct current. U. S. Fish Wildl. Serv., Fish. <br />Bull. 56: 61-81. <br />DELOv, V. E, AND I. F. ToMASHEVSKU. 1933. Prob- <br />lema elektricheskogo ]ova ryby (Problem of elec- <br />tro-fishing). Irv. Vses. Nauch: issled. Inst. Ozer. <br />Rechn. Ryb. Khoz. 16: 5-17. (Trans]. by Int. <br />Pac. Halibut Comm., Fisheries Hall No. 2, Uni- <br />versity of Washington, Seattle.) <br />GIGUERE, PAUL E. 1954. Development and use of <br />electro-fishing apparatus in Region V. Calif. <br />Dept Fish Game, Inland Fish. Br., Admin. Rept. <br />5417 34 pp. (Processed.) <br />HORAK, DONALD L., AND WILLIAM D. KLEIN. 1967. <br />Influence of capture methods on fishing success, <br />stamina, and mortality of rainbow trout (Salmo <br />gairdneri) in Colorado. Trans. Amer. Fish. Soc. <br />96(2): 220-222. <br />KLEIN, W. D. 1967. Evaluation of a pulsating direct- <br />current shocking device for obtaining trout from <br />a lake for population estimates. Progr. Fish-Cult. <br />29: 140-149. <br />AND L. M. FINNELL. 1969. Comparative <br />study of coho salmon introductions in Parvin <br />Lake and Granby Reservoir. Progr. Fish-Cult. <br />31: 99-108. <br />KUROKI, T. 1959. Electrical fishing in Japan. In: <br />Hilmar Kristionsson (ed.), Modern fishing gear <br />of the world, pp. 581-582. Fishing News (Books) <br />Ltd., London. <br />LOWRY, GERALD R. 1966. Production and food of <br />cutthroat trout in three Oregon coastal streams. <br />J. Wild]. Management 30(4) : 754-767. <br />McLAIN, ALBERTON L. 1957. The control of the <br />upstream movement of fish with pulsated direct <br />current. Trans. Amer. Fish. Soc. 86: 269-284. <br />AND WILLIS L. NIELSEN. 1953. Directing the <br />movement of fish with electricity. U. S. Fish <br />Wildl. Serv., Spec. Sci. Rept. Fish. 93, 24 pp. <br />MCMILLAN, F. O. 1928. Electric fish screen. U. S. <br />Bur. Fish., Bull. 44: 97-128. <br />MAXFIELD, GALEN H., GERALD E. MONAN, AND HOL- <br />BROOK L. GARRETT. 1969. Electrical installation <br />for control of the northern squaw-fish. U. S. Fish <br />Wildl. Serv., Spec. Sci. Rept. Fish. 583, 14 pp. <br />MEYER-WAARDEN, [P. F.J. 1953. Beeinflusst die Elek, <br />tronarkose Lebensf ihigkeit and Wachstum der <br />Fische? (Does electronarcosis influence the vi- <br />tality and growth of fish?). Der Fischwirt 3(7) <br />225-228. (Transl., Bur. Commer. Fish., Biol. <br />Lab., Seattle, Washington, 1969.) <br />MILLER, DENNY MARVIN. 1%5. Evaluation of the <br />suitability for salmonid production of a stream <br />with multiple water uses. M.S. Thesis, University <br />Of Washington, Seattle, vii -{- 88 pp. (Processed.) <br />MOORE, W. H. 1968. A light-weight pulsed d.c. fish <br />shocker. J. Appl. Ecol. 5(1): 205-208. <br />RIEDEL, DIETMAR. 1952. Uber efne Beeinflussung der <br />Fischgeschlechtsprodukte durch den Elektrischen <br />Strom unter besonderer Beriieksichtigung der <br />Elektrofiscberei (The effect of electrical current <br />on the sexual products of fish with special em- <br />phasis on electro-fishing). Inaugural dissertation, <br />Doktor der Landwirtschaft, Humboldt University, <br />Berlin, No. 819, 53 pp. Also published 1954, <br />Z. Fisch. deren Hilfswiss. 3(1/2/3): 183-233. <br />(Trans]. of dissertation, Bur. Commer. Fish., Biol. <br />Lab., Seattle, Washington.) <br />SHARPE, F. PHILLIP. 1964. An electrofishing boat <br />with a variable voltage pulsator for lake and <br />reservoir studies. Bur. Sport Fish. Wild]., Circ. <br />195, 6 pp. <br />SMITH, LLOYD L., JR., DONALD R. FRANKLIN, AND <br />ROBERT H. KRAMER. 1959. Electro-fishing for <br />small fish in lakes. Trans. Amer. Fish. Sue. <br />88(2): 141-146. <br />An Evaluation of the Use of a Basket-Type Artificial <br />Substrate for Sampling Macroinvertebrate Organisms <br />KENNETH L. DICKSON,' JOHN CAIRNS, JR.,' AND JESSE C. ARNOLD2 <br />Virginia Polytechnic Institute and State University <br />Blacksburg, Virginia 24061 <br />ABSTRACT <br />The results obtained from the use of bottom, basket type, artificial samplers, were analyzed <br />statistically to determine the samplers' efficiency in collecting aquatic macroinvertebrates. Two <br />ecologically similar riffle stations located near McCoy, Virginia on the New River were chosen <br />for this evaluation. Based on an analysis of the number of taxa collected, 15 basket samplers <br />at Station 1 showed a range of seven taxa with a coefficient of variation of 11.6%. Station 2, <br />located 500 yards upstream, with 16 samplers, had a range of 11 taxa with a coefficient of <br />variation of 16.297c. In an evaluation of the number of specimens collected, Station 1 had a <br />range of 39 specimens with a coefficient of variation of 10.9%. Station 2 had a range of 103 <br />specimens which was larger than the average of 85.6 specimens per sampler. To be 95ofo confident <br />that the mean community structure indices for each station [Sequential Comparison Index and <br />diversity per individual (d) ] were within 251% of their true value, only two basket samplers were <br />needed. However, four samplers at Station 1 and six samplers at Station 2 would be required <br />for the number of taxa collected Due to unequal distribution of macroinvertebrates on the <br />bottom, or sampling design bias, 21 samplers at Station 2 would be required to be 95% confident <br />that the mean number of specimens collected was within 25% of its true value. An estimate of <br />the proportion of taxa which would have been detected on the average by a smaller number of <br />basket samplers showed that at least five basket samplers were necessary at each station in <br />order to be 95% confident that at least 50°fo of the total number of taxa were collected. When <br />using this type of substrate sampler for collecting macroinvertebrates in a comparison of the <br />biological water quality between selected stations, the number of taxa collected by the samplers <br />and the community structure of the macroinvertebrates are less variable than the number of <br />specimens obtained. These types of analyses can serve as the basis for establishing the appropriate <br />number of samplers for a routine survey. <br />INTRODUCTION <br />In the process of trying to develop routine <br />biological water pollution monitoring pro- <br />grams for industry, we found that very few <br />data were available in the literature which <br />could be used to evaluate the reliability of <br />artificial substrate samplers for collecting <br />macroinvertebrate organisms. Accordingly, it <br />was decided that field tests would be conducted <br />to determine the normal variability in the <br />macroinvertebrates collected by replicate bot- <br />tom basket-type artificial substrate samplers <br />placed in a riffle. <br />The use of artificial substrate samplers to <br />collect macroinvertebrate organisms is increas- <br />ing in the biological assessment of water <br />quality. The Division of Pollution Surveil- <br />lance of the Environmental Protection Agency <br />uses a modified barbeque basket filled with <br />limestone as an artificial substrate sampler in <br />'Biology Department <br />'Statistics Department. <br />its routine surveillance program (Mason et al., <br />1967; Anderson and Mason, 1968). <br />A variety of different types of artificial <br />substrate devices have been developed and <br />employed in the collection of the macroinverte. <br />brates which inhabit the benthic areas of both <br />lentic and lotic series. These vary in construc- <br />tion from the simple concrete slabs of Moon <br />(1935) and the hardware cloth "brush boxes" <br />of Wene and Wickliff (1940) and Scott (1958) <br />to multiple-plate artificial substrate samplers <br />such as those-described by Hester and Dendy <br />(1962). By incorporating a sample-catcher, <br />Hilsenhoff (1969) developed an artificial sub- <br />strate device for collecting stream invertebrates <br />which prevents loss of organisms during re- <br />moval of the sampler from the water. <br />Artificial substrates have many advantages <br />over conventional sampling techniques for <br />collecting macroinvertebrates. Using artificial <br />substrates, it is possible to sample large <br />streams which have beds of large rocks or of <br />shifting substrates, both of which are virtually <br />impossible to sample with any reliability using <br />553