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136 The Southwestern Naturalist vol. 30, no. I <br />within the chorion. Time to peak hatch decreased with increased tempera- <br />ture from 156 It (15°C) to 72 hrs (25°C). Newly hatched normal prolarvae <br />were longest at 15°C (63 mm) compared with 5.5 and 5.7 mm at 20 and <br />25°C respectively. Behavior of hatched prolarvae was the same as for bony- <br />tail. Swimup occurred at 372 h at 151C to 166 h at 25°C, depending on <br />temperature (Table 4). Swimup fry were 8.2-8.5 mm at 20 and 25°C; no <br />lengths were obtained for swimup fry at 151C. Incidence of abnormal fry <br />was significantly greater at 15°C (33%) than at 20 or 25°C (13% and 17%, <br />respectively). <br />Colorado squawfish.-Percentage hatch of squawfish was 27% at 20°C in <br />trial I, and 2% (20°C) and 9% (251C) in trial II (Table 4). All ova died at 5-15, <br />25, and 30°C (trial 1) or 5-15, and 30°C (trial II). As with other species, most <br />mortality occurred before embryo motility was attained. Peak hatch was at <br />78-108 h at 20°C (both trials) compared with 63 h at 25'C. <br />Hatched normal prolarvae averaged 5.5 mm at 20°C and 5.6 mm at 25°C. <br />Prolarvae were seemingly helpless at hatching, moving feebly on the bot- <br />tom. However, larvae reacted to bright light by frantic, apparently undi- <br />rected swimming activity, which ceased within 15-30 s after removal of the <br />light source. This increased activity also was observed in prehatch embryos, <br />which vibrated within the chorion when exposed to bright light under a <br />microscope. Abnormal fry accounted for 26% of total hatch at 25°C more <br />than twice the 11% incidence at 20°C (Table 4). <br />Development Rates.-Development rate (V, Table 4) at each temperature <br />was similar for all species, 4.4-6.1 (15°C), 8.4-9.9 (20°C), and 12.4-18.8 (25°C). <br />Yet, linear development rate versus temperature function slopes for pairwise <br />species comparisons were significantly different (F-test for equality of slope, <br />p < 0.05) between Colorado squawfish and razorback sucker, humpback <br />chub and razorback sucker, and between Colorado squawfish and bonytail <br />chub. These comparisons likely reflect basic differences in developmental <br />physiology among species. <br />DISCUSSION.-Results herein are generally consistent with earlier work <br />(Toney, 1974; Inslee, 1982; Harriman, 1981a-b, 1982a-b; Bulkley et al., 1981), <br />clearly demonstrate that survival and hatch are maximum near 20°C and <br />show that mortality increases dramatically with < 10°C change from that <br />temperature. Potential effects of adult acclimation temperature upon embryo <br />survival and hatch at various incubation temperatures cannot be assessed, <br />although effects have been noted with some species (e.g., Koenst and Smith, <br />1976) and might reasonably be expected (Brett, 1956). <br />Although every attempt was made to standardize variables other than <br />temperature, factors remain which may have influenced results: fungus on <br />devloping egges, health and condition of broodfish, differences between <br />wild-caught and hatchery-reared or maintained individuals, manipulation <br />and handling, induced spawning as compared with "natural ripening" of <br />broodfish, and unknown differences between the experimental system and <br />natural incubation conditions. These could influence absolute values of sur- <br />vival or percentage hatch at a given temperature, but should have influenced <br />relative relationships (differences in percentage hatch among temperatures)