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or high velocity as the other main effect. <br />RESULTS <br />Substrate Experiments <br />There were three substrate types available for each replicate. In the first set of trials <br />(sand, gravel, and sand/gravel replicates), substrate preference was not independent of <br />the time of observation (e.g. day versus night) as determined by a R x C test (x2=21.88, <br />df=2, p< 0.001). During the day, bonytail chose gravel 50% of the time, sand 30% and <br />sand/gravel 20% (Fig. 1.1). However, at night bonytail chose gravel 34% of the time, <br />sand 40% and sand/gravel 26%. <br /> <br />? 50 <br /> ? Night <br />? Day <br />L <br /> 40 <br /> <br /> <br />O 30 <br />.F+ <br /> <br /> 20 <br />V <br />L <br />Q 10 <br />M <br /> 0 <br />Sand Sand & Gravel Gravel <br />Substrate Treatment <br />Figure 1.1 Substrate selection by bonytail without red shiners present (low flow). <br />In trials with sand, gravel, and cobble as available substrates, substrate selection was <br />independent of the time of observation (x2=0, df=2, p=0.999). In these replicates, <br />bonytail showed a much more significant preference for substrate, selecting for cobble <br />substrate 100% of the time; there was no variance (Fig. 1.2) <br />15